JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327160

Anti-POU3F3 Antibody [CL15308]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal POU3F3/Brain1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human POU3F3.

View Alternative Names

BRN1, OTF8, POU3F3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8, Brain-1, Brn-1, Oct-8, OTF-8

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemistry analysis in human kidney and skeletal muscle tissues using ab327160 at 1 : 1000–1 : 2500 dilution. Corresponding POU3F3 RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of human kidney using ab327160 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in cells in tubules.

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunofluorescence staining in SH-SY5Y cell line using ab327160 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of human cerebellum using ab327160 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in granular layer and molecular layer cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of human cerebral cortex using ab327160 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in neurons and glial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of mouse embryo, E14 using ab327160 at 1 : 1000–1 : 2500 dilution, showing strong nuclear positivity in neurons in developing cerebral cortex.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of human skeletal muscle using ab327160 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU3F3 Antibody [CL15308] (AB327160)

Immunohistochemical staining of human rectum using ab327160 at 1 : 1000–1 : 2500 dilution, showing no positivity in glandular cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL15308

Isotype

IgG2b

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human POU3F3.

P20264

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development (PubMed : 31303265). Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).
See full target information POU3F3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com