Rabbit Polyclonal POU5F1B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- Cancers 15:2023Dual Function of CCAT2 in Regulating Luminal Subtype of Breast Cancer Depending on the Subcellular Distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeying Xie,Yuefan Guo,Zhen Xu,Qiong Wang,Tao Wang,Yi Gu,Danni Li,Yu Liu,Wenjing Ma,Pengfei Liu,Qian Zhao,Jinhui Lü,Junjun Liu,Zuoren YuPubMed 36672487
- BioMed research international 2021:54050602021Recurrent Superenhancer of the Oncogene in Colorectal Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Chuan Tao,Cheng Wang,Ning Ma,Xun Zhu,Xiao-Jun ZhouPubMed 34934770
- RNA biology 18:369-3812021LncRNA CASC21 induces HGH1 to mediate colorectal cancer cell proliferation, migration, EMT and stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxin Zhang,Jifu E,Enda YuPubMed 34375566
- Annals of translational medicine 9:10162021Beta-1 syntrophin (SNTB1) regulates colorectal cancer progression and stemness via regulation of the Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanfang Liang,Bin Wang,Shasha Chen,Ziyu Ye,Xingxing Chai,Ronggang Li,Xiaoping Li,Gang Kong,Yanyun Li,Xueying Zhang,Zhengping Che,Qi Xie,Jiachun Lian,Bihua Lin,Xin Zhang,Xueqin Huang,Weijuan Huang,Xianxiu Qiu,Jincheng ZengPubMed 34277816
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q06416
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
Target data
Function
Shows weak transcriptional activator activity.
Alternative names
OCT4PG1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU5F1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like
Recommended products
Rabbit Polyclonal POU5F1B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q06416
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-POU5F1B antibody (ab230429)
All lanes: Western blot - Anti-POU5F1B antibody (ab230429) at 1/1000 dilution
All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU5F1B antibody (ab230429)
Formalin-fixed, paraffin-embedded human testis tissue stained for POU5F1B using ab230429 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry (Intracellular) - Anti-POU5F1B antibody (ab230429)
Intracellular flow cytometric analysis ofK562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling POUF5F1B with ab230429 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com