AB230429

Anti-POU5F1B antibody

20ul selling size
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(6 Publications)

Rabbit Polyclonal POU5F1B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

OCT4PG1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU5F1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like

3 Images

Flow Cytometry (Intracellular) - Anti-POU5F1B antibody (AB230429)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-POU5F1B antibody (AB230429)

Intracellular flow cytometric analysis ofK562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling POUF5F1B with ab230429 at 1/10 dilution (green) compared with a negative control (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU5F1B antibody (AB230429)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU5F1B antibody (AB230429)

Formalin-fixed, paraffin-embedded human testis tissue stained for POU5F1B using ab230429 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-POU5F1B antibody (AB230429)

All lanes:

Western blot - Anti-POU5F1B antibody (ab230429) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human POU5F1B aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q06416

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Shows weak transcriptional activator activity.

See full target information POU5F1B

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Folia histochemica et cytobiologica 63:11-27 PubMed40152123

2025

Inhibition of STAT1 alleviates oxidative damage in retinal pigment epithelial cells and exhibits neuroprotective effects in autoimmune optic neuritis by upregulating IFI30 lysosomal thiol reductase.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Ma,Jiajia Yuan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308823 PubMed39287146

2024

VPS34 Governs Oocyte Developmental Competence by Regulating Mito/Autophagy: A Novel Insight into the Significance of RAB7 Activity and Its Subcellular Location.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Liu,Kehan Wang,Yuting Lin,Lu Wang,Xin Jin,Yuexin Qiu,Wenya Sun,Ling Zhang,Yan Sun,Xiaowei Dou,Shiming Luo,Youqiang Su,Qingyuan Sun,Wenpei Xiang,Feiyang Diao,Jing Li

Cancers 15: PubMed36672487

2023

Dual Function of CCAT2 in Regulating Luminal Subtype of Breast Cancer Depending on the Subcellular Distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Heying Xie,Yuefan Guo,Zhen Xu,Qiong Wang,Tao Wang,Yi Gu,Danni Li,Yu Liu,Wenjing Ma,Pengfei Liu,Qian Zhao,Jinhui Lü,Junjun Liu,Zuoren Yu

BioMed research international 2021:5405060 PubMed34934770

2021

Recurrent Superenhancer of the Oncogene in Colorectal Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Chuan Tao,Cheng Wang,Ning Ma,Xun Zhu,Xiao-Jun Zhou

RNA biology 18:369-381 PubMed34375566

2021

LncRNA CASC21 induces HGH1 to mediate colorectal cancer cell proliferation, migration, EMT and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxin Zhang,Jifu E,Enda Yu

Annals of translational medicine 9:1016 PubMed34277816

2021

Beta-1 syntrophin (SNTB1) regulates colorectal cancer progression and stemness via regulation of the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfang Liang,Bin Wang,Shasha Chen,Ziyu Ye,Xingxing Chai,Ronggang Li,Xiaoping Li,Gang Kong,Yanyun Li,Xueying Zhang,Zhengping Che,Qi Xie,Jiachun Lian,Bihua Lin,Xin Zhang,Xueqin Huang,Weijuan Huang,Xianxiu Qiu,Jincheng Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-POU5F1B antibody

Flow Cytometry (Intracellular) - Anti-POU5F1B antibody (AB230429)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU5F1B antibody (AB230429)

Western blot - Anti-POU5F1B antibody (AB230429)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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