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AB185209

Anti-PP11 antibody [EPR15137] - N-terminal

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(1 Publication)

Rabbit Recombinant Monoclonal PP11 antibody. N-terminal. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Uridylate-specific endoribonuclease, Placental protein 11, Protein endoU, PP11, ENDOU

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)

Immunohistochemical analysis of Paraffin-embedded Human placenta tissue labeling PP11 with ab185209 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)
  • IP

Supplier Data

Immunoprecipitation - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)

Western blot analysis of immunoprecipitation pellet from Human fetal skin lysate immunoprecipitated using ab185209 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)

Predicted band size: 47 kDa

false

Western blot - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)
  • WB

Supplier Data

Western blot - Anti-PP11 antibody [EPR15137] - N-terminal (AB185209)

Based on the sequence analysis, ab185209 recognizes two isoforms with the predicted MWs of 42KDa and 47KDa, respectively.

All lanes:

Western blot - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209) at 1/10000 dilution

Lane 1:

Human placenta lysate at 20 µg

Lane 2:

Human skin lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false

  • Carrier free

    Anti-PP11 antibody [EPR15137] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15137

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoribonuclease that cleaves single-stranded RNAs at 5' of uridylates and releases a product with a 2',3'-cyclic phosphate at the 3'-end. The UU and GU sites are more efficiently cleaved than CU and AU sites (PubMed : 18936097). Targets mRNAs to regulate their expression (PubMed : 37803019).
See full target information ENDOU
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:3472-3488 PubMed33537098

2021

Overall survival of pancreatic ductal adenocarcinoma is doubled by deletion in the KPC mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Seon Lee,Ho Lee,Sang Myung Woo,Hyonchol Jang,Yoon Jeon,Hee Yeon Kim,Jaewhan Song,Woo Jin Lee,Eun Kyung Hong,Sang-Jae Park,Sung-Sik Han,Soo-Youl Kim
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com