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AB188253

Anti-PP2A-2 catalytic subunit antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal PP2A-2 catalytic subunit antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Schizosaccharomyces pombe samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Schizosaccharomyces pombe 972h- ppa2.

View Alternative Names

SPBC16H5.07c, ppa2, Major serine/threonine-protein phosphatase PP2A-2 catalytic subunit

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PP2A-2 catalytic subunit antibody - C-terminal (AB188253)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PP2A-2 catalytic subunit antibody - C-terminal (AB188253)

Immunofluorescence analysis of S.pombe cells, labeling PPA2 with ab188253 (right). DNA staining with DAPI (left). 1. ΔPPA2. 2. wild-type. 3. wild-type overexpressing PPA2 (nmt-PPA2, 18hr). Bar, 10 μm.

Western blot - Anti-PP2A-2 catalytic subunit antibody - C-terminal (AB188253)
  • WB

Supplier Data

Western blot - Anti-PP2A-2 catalytic subunit antibody - C-terminal (AB188253)

The positions of PPA1 (36 kDa) and PPA2 (39 kDa) polypeptide bands are indicated.

All lanes:

Western blot - Anti-PP2A-2 catalytic subunit antibody - C-terminal (ab188253) at 1/1000 dilution

Lane 1:

Wild-type S. pombe cell extract

Lane 2:

ΔPPA1

Lane 3:

ΔPPA2

Lane 4:

Wild-type carrying a multicopy plasmid with PPA1 gene

Lane 5:

Wild-type carrying a multicopy plasmid with PPA2 gene

Lane 6:

Wild-type carrying a multicopy plasmid with ADH promoter ligated with PPA2 gene

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Schizosaccharomyces pombe

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Schizosaccharomyces pombe 972h- ppa2. The exact immunogen used to generate this antibody is proprietary information.

P23636

Specificity

ab188253 recognized both PPA1 and PPA2 polypeptides in S. pombe because of their high amino acid similarity (~80% identity).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Schizosaccharomyces pombe": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential role in cell cycle control. PP2A may be involved in controlling the entry into mitosis, possibly acting as an inhibitor.
See full target information ppa2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:831 PubMed40993725

2025

USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Liu,Jin Zhang,Jichang Bai,Kuanzhi Liu
View all publications
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Product promise

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