Rabbit Polyclonal PP2A-alpha antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PPP2CA aa 1-50.
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- Frontiers in pharmacology 13:8172132022Chinese Herbal Extracts Exert Neuroprotective Effect in Alzheimer's Disease Mouse Through the Dopaminergic Synapse/Apoptosis Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Huang,Chen Zhang,Sihao Qu,Shi Dong,Qihong Ma,Ying Hao,Zimin Liu,Shanglong Wang,Haibin Zhao,Yuanyuan ShiPubMed 35295332
- The FEBS journal 289:4518-45352022Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Gao,Xin Li,Huiying Gao,Ke Zhao,Xian Liu,Jinfang Liu,Qi Wang,Yaxin Zhu,Hui Chen,Shensi Xiang,Yiqun Zhan,Ronghua Yin,Miao Yu,Hongmei Ning,Xiaoming Yang,Changyan LiPubMed 35068054
- Journal of gastrointestinal oncology 12:3008-30212022The effect of expression on the prognosis of patients with hepatocellular carcinoma and its molecular biological characteristics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingchang Liang,Yu Huang,Chenglei Yang,Shen Huang,Jinlong Xie,Xiang Nong,Jianyong Liu,Yumei Zhang,Zhiming ZhangPubMed 35070426
- JCI insight 6:2021Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingmiao Lu,Qing Hou,Kai Cao,Xiaoli Sun,Yan Liang,Mengru Gu,Xian Xue,Allan Zijian Zhao,Chunsun DaiPubMed 34622800
- Journal of clinical and translational hepatology 9:661-6712021Potential Role and Clinical Value of PPP2CA in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Lei Yang,Xue Qiu,Jin-Yan Lin,Xiao-Yu Chen,Yu-Mei Zhang,Xiao-Yin Hu,Jian-Hong Zhong,Shen Tang,Xi-Yi Li,Bang-De Xiang,Zhi-Ming ZhangPubMed 34722181
- Cell death and differentiation 28:2728-27442021PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Liang,Xiaoli Sun,Mingjie Wang,Qingmiao Lu,Mengru Gu,Lu Zhou,Qing Hou,Mengzhu Tan,Sudan Wang,Xian Xue,Chunsun DaiPubMed 33934104
- Cell 181:688-701.e162020Selective PP2A Enhancement through Biased Heterotrimer Stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Leonard et. al.PubMed 32315618
- Cell reports 30:3164-3170.e32020Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr on PP2Ac.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSahar Mazhar,Daniel Leonard,Alejandro Sosa,Daniela Schlatzer,Dafydd Thomas,Goutham NarlaPubMed 32130915
- PloS one 14:e02144692019PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura L Stafman,Adele P Williams,Raoud Marayati,Jamie M Aye,Jerry E Stewart,Elizabeth Mroczek-Musulman,Elizabeth A BeierlePubMed 30969990
- The Journal of clinical investigation 129:1641-16532019RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaikamjad Umesalma,Courtney A Kaemmer,Jordan L Kohlmeyer,Blake Letney,Angela M Schab,Jacqueline A Reilly,Ryan M Sheehy,Jussara Hagen,Nitija Tiwari,Fenghuang Zhan,Mariah R Leidinger,Thomas M O'Dorisio,Joseph Dillon,Ronald A Merrill,David K Meyerholz,Abbey L Perl,Bart J Brown,Terry A Braun,Aaron T Scott,Timothy Ginader,Agshin F Taghiyev,Gideon K Zamba,James R Howe,Stefan Strack,Andrew M Bellizzi,Goutham Narla,Benjamin W Darbro,Frederick W Quelle,Dawn E QuellePubMed 30721156
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPP2CA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P67775
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-2 µg/mL | Notes - |
Species Rat | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Boiling the sections is required. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed:22613722). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed:22613722). Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53 (PubMed:17245430). Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758). Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed:25438055). Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed:30513302). Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:33633399).
Alternative names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Recommended products
Rabbit Polyclonal PP2A-alpha antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PPP2CA aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPP2CA aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P67775
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-PP2A-alpha antibody (ab106262)
All lanes: Western blot - Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/mL
Lane 1: Rat kidney tissue Lysate at 40 µg
Lane 2: Rat Liver tissue Lysate at 40 µg
Lane 3: Human Placenta tissue Lysate at 40 µg
Lane 4: Mouse Kidney tissue Lysate at 40 µg
Lane 5: A431 Whole Cell Lysate at 40 µg
Lane 6: HeLa Whole Cell Lysate at 40 µg
Lane 7: 293T Whole Cell Lysate at 40 µg
Lane 8: NIH Whole Cell Lysate at 40 µg
Lane 9: PC12 Whole Cell Lysate at 40 µg
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody (ab106262)
Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of human mammary cancer, using ab106262 at a concentration of 2μg/ml.
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