AB137825
Rabbit Polyclonal PP2A-alpha antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP2CA aa 1-250.
View Alternative Names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
4 Images
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody (AB137825)
Confocal immunofluorescence analysis of methanol fixed HeLa cells, labelling PP2A alpha with ab137825 (Green) at 1/500 dilution. Alpha tubulin filaments were labelled with an alpha tubulin antibody (Red) at 1/2000.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody (AB137825)
Immunohistochemical analysis of paraffin embedded Cal27 xenograft, labelling PP2A alpha with ab137825 at 1/100 dilution.
- WB
Supplier Data
Western blot - Anti-PP2A-alpha antibody (AB137825)
Samples were separated by 10% SDS PAGE.
All lanes:
Western blot - Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution
Lane 1:
A549 whole cell extracts at 30 µg
Lane 2:
H1299 whole cell extracts at 30 µg
Lane 3:
HCT116 whole cell extracts at 30 µg
Secondary
All lanes:
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa
false
- WB
Unknown
Western blot - Anti-PP2A-alpha antibody (AB137825)
10% SDS PAGE
All lanes:
Western blot - Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution
All lanes:
Mouse brain whole cell lysate at 50 µg
Predicted band size: 35 kDa
false
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Reactivity data
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Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data
Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (PubMed : 10801873, PubMed : 12473674, PubMed : 17245430, PubMed : 22613722, PubMed : 33243860, PubMed : 34004147, PubMed : 9920888). PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed : 22613722). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed : 22613722). Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate various proteins, such as SV40 large T antigen, AXIN1, p53/TP53, PIM3, WEE1 (PubMed : 10801873, PubMed : 12473674, PubMed : 17245430, PubMed : 9920888). Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed : 10801873). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed : 33108758). Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis : interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed : 25438055). Mediates dephosphorylation of FOXO3; promoting its stabilization : interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed : 30513302). Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome (By similarity). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Catalyzes dephosphorylation of PIM3, promoting PIM3 ubiquitination and proteasomal degradation (PubMed : 12473674). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 33633399). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling (PubMed : 33633399). Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 33633399). Key mediator of a quality checkpoint during transcription elongation as part of the Integrator-PP2A (INTAC) complex (PubMed : 33243860, PubMed : 34004147, PubMed : 37080207). The INTAC complex drives premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : within the INTAC complex, PPP2CA catalyzes dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, thereby preventing transcriptional elongation (PubMed : 33243860, PubMed : 34004147, PubMed : 37080207).
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in nutrition 9:892558 PubMed35662922
2022
Inhibitory Effects of Macelignan on Tau Phosphorylation and Aβ Aggregation in the Cell Model of Alzheimer's Disease.
Applications
Unspecified application
Species
Unspecified reactive species
Liang Gu,Nan Cai,Meiting Li,Decheng Bi,Lijun Yao,Weishan Fang,Yan Wu,Zhangli Hu,Qiong Liu,Zhijian Lin,Jun Lu,Xu Xu
ASN neuro 13:1759091420984920 PubMed33430619
2021
Role of G-Substrate in the NO/cGMP/PKG Signal Transduction Pathway for Photic Entrainment of the Hamster Circadian Clock.
Applications
Unspecified application
Species
Unspecified reactive species
Santiago Andrés Plano,María Soledad Alessandro,Laura Lucía Trebucq,Shogo Endo,Diego Andrés Golombek,Juan José Chiesa
Cell death and differentiation 27:2952-2972 PubMed32424140
2020
The proteasome activator REGγ accelerates cardiac hypertrophy by declining PP2Acα-SOD2 pathway.
Applications
Unspecified application
Species
Unspecified reactive species
Yifan Xie,Yang Gao,Rifeng Gao,Wenlong Yang,Zheng Dong,Robb E Moses,Aijun Sun,Xiaotao Li,Junbo Ge
Molecular medicine reports 14:1247-54 PubMed27279479
2016
Anxiolytic and neuroprotective effects of the Traditional Chinese Medicinal formulation Dan-zhi-xiao-yao-san in a rat model of chronic stress.
Applications
Unspecified application
Species
Unspecified reactive species
Guo-Ping Cao,Dan Gui,Lu-Di Fu,Zhou-Ke Guo,Wen-Jun Fu
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