AB313851

Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal PP2A-alpha antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat, Cat, Dog, Drosophila melanogaster samples. Immunogen corresponding to Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Ppp2ca, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Ppp2ca

10 Images

Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunofluorescent analysis of A431 (Human epidermoid carcinoma cell line) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling PP2A-alpha with ab313851 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.

Supplier Data

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/20000 dilution

Lane 1:

Non-transfected (–) 293T cells at 30 µg

Lane 2:

PP2A alpha shRNA-transfected 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

All lanes:

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

All lanes:

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 30 µg

Lane 2:

Rat2 (Rat fibroblast cell line) cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemical analysis of paraffin-embedded dog kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemical analysis of paraffin-embedded cat kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/1000 dilution

All lanes:

S2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1447

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Cat, Dog, Drosophila melanogaster

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.

P67775

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (PubMed : 10801873, PubMed : 12473674, PubMed : 17245430, PubMed : 22613722, PubMed : 33243860, PubMed : 34004147, PubMed : 9920888). PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed : 22613722). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed : 22613722). Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate various proteins, such as SV40 large T antigen, AXIN1, p53/TP53, PIM3, WEE1 (PubMed : 10801873, PubMed : 12473674, PubMed : 17245430, PubMed : 9920888). Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed : 10801873). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed : 33108758). Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis : interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed : 25438055). Mediates dephosphorylation of FOXO3; promoting its stabilization : interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed : 30513302). Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome (By similarity). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Catalyzes dephosphorylation of PIM3, promoting PIM3 ubiquitination and proteasomal degradation (PubMed : 12473674). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 33633399). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling (PubMed : 33633399). Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 33633399). Key mediator of a quality checkpoint during transcription elongation as part of the Integrator-PP2A (INTAC) complex (PubMed : 33243860, PubMed : 34004147, PubMed : 37080207). The INTAC complex drives premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : within the INTAC complex, PPP2CA catalyzes dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, thereby preventing transcriptional elongation (PubMed : 33243860, PubMed : 34004147, PubMed : 37080207).

See full target information PPP2CA

Additional targets

Ppp2ca,Ppp2ca

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Additional targets

Product promise