Rabbit Recombinant Monoclonal PP2A-alpha antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat, Cat, Dog, Drosophila melanogaster samples. Immunogen corresponding to Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.
Images
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441362.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441363.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441364.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429411.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin. Database link P67775
Reactivity data
Select an application| ICC/IF | IHC-P | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Tested |
| Cat | Expected | Tested | Expected |
| Dog | Expected | Tested | Expected |
| Drosophila melanogaster | Expected | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Dog, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Dog | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Drosophila melanogaster | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed:22613722). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed:22613722). Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53 (PubMed:17245430). Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758). Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed:25438055). Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed:30513302). Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:33633399).
Additional Targets
Ppp2ca, Ppp2ca
Alternative names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Recommended products
Rabbit Recombinant Monoclonal PP2A-alpha antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat, Cat, Dog, Drosophila melanogaster samples. Immunogen corresponding to Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin. Database link P67775
- Carrier free
- Yes
- Clone number
- HL1447
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441362.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Samples were seperated by 10% SDS-PAGE.
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/20000 dilution
Lane 1: Non-transfected (–) 293T cells at 30 µg
Lane 2: PP2A alpha shRNA-transfected 293T cells at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 35 kDa
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441363.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Samples were seperated by 10% SDS-PAGE.
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution
Lane 1: Neuro2A whole cell extracts at 30 µg
Lane 2: Rat2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 35 kDa
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img441364.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Samples were seperated by 10% SDS-PAGE.
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/1000 dilution
All lanes: S2 Whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429411.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded dog kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded cat kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunocytochemistry-immunofluorescence-img418894.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunofluorescent analysis of A431 (Human epidermoid carcinoma cell line) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling PP2A-alpha with ab313851 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418875.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418874.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418907.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418906.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.
![Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunocytochemistry-immunofluorescence-img418894.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418875.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418874.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418907.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418906.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
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