Rabbit Recombinant Monoclonal PP2A-alpha antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat, Cat, Dog samples. Immunogen corresponding to Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429411.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunocytochemistry-immunofluorescence-img418894.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418875.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418874.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (AB313851)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin. Database link P67775
Reactivity data
Select an application| ICC/IF | IHC-P | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Tested |
| Cat | Expected | Tested | Expected |
| Dog | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Dog | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758).
Targets
Ppp2ca, Ppp2ca
Alternative names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Recommended products
Rabbit Recombinant Monoclonal PP2A-alpha antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat, Cat, Dog samples. Immunogen corresponding to Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin.
Alternative names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human PPP2CA conjugated to Keyhole Limpet Haemocyanin. Database link P67775
- Carrier free
Yes
- Clone number
HL1447
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429411.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded dog kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded cat kidney tissue labelling PP2A-alpha with ab313851 at 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunocytochemistry-immunofluorescence-img418894.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunocytochemistry/ Immunofluorescence - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunofluorescent analysis of A431 (Human epidermoid carcinoma cell line) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling PP2A-alpha with ab313851 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418875.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) cell lysate at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa
![Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--western-blot-img418874.jpg "Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
All lanes: Western blot - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851) at 1/2000 dilution
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418907.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418906.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PP2A alpha with ab313851 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418907.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851), expandable thumbnail](https://content.abcam.com/products/images/pp2a-alpha-antibody-hl1447-bsa-and-azide-free-ab313851--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418906.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody [HL1447] - BSA and Azide free (ab313851)")
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