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Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-PPA2 antibody [EPR13086] (AB180859), expandable thumbnail
  • Western blot - Anti-PPA2 antibody [EPR13086] (AB180859), expandable thumbnail
  • Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (AB180859), expandable thumbnail
  • Western blot - Anti-PPA2 antibody [EPR13086] (AB180859), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (AB180859), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Not recommended
Mouse
Expected
Expected
Not recommended
Tested
Not recommended
Rat
Expected
Expected
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/50 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/50 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Species
Rat
Dilution info
1/10 - 1/100
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Hydrolyzes inorganic pyrophosphate (PubMed:27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed:27523598).

Alternative names

HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2

Recommended products

Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13086
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 10 µg

    Lane 2: Rat heart tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (ab180859) at 1/10000 dilution

    All lanes: HepG2 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa

    Observed band size: 35 kDa

  • Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

    ab180859 (purified) at 1/80 immunoprecipitating PPA2 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10μg)

    Lane 2 (+): ab180859 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab180859 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

    Predicted band size: 38 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution

    All lanes: 293T whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa

    Observed band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with purified ab180859 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

    All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution

    Lane 1: Human fetal kidney tissue lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: U-87 MG cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Predicted band size: 38 kDa

  • Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

    Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180859 at a diltuion of 1/10.

    All lanes: Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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