Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)

Rabbit Recombinant Monoclonal PPA2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (AB250259), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Not recommended	Tested	Not recommended
Mouse	Expected	Expected	Not recommended	Tested	Not recommended
Rat	Expected	Expected	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information PPA2

Function

Hydrolyzes inorganic pyrophosphate (PubMed:27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed:27523598).

Alternative names

HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2

Recommended products

Rabbit Recombinant Monoclonal PPA2 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR13086
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab250259 is the carrier-free version of Anti-PPA2 antibody [EPR13086] ab180859.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 10 µg
Lane 2: Rat heart tissue lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 35 kDa
Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859) at 1/10000 dilution
All lanes: HepG2 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 35 kDa
Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.
Anti-PPA2 antibody [EPR13086] ab180859 (purified) at 1/80 immunoprecipitating PPA2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10Âμg).
Lane 2 (+): Anti-PPA2 antibody [EPR13086] ab180859 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PPA2 antibody [EPR13086] ab180859 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859)
Predicted band size: 38 kDa
Observed band size: 35 kDa
Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859) at 1/1000 dilution
All lanes: 293T whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with purified Anti-PPA2 antibody [EPR13086] ab180859 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859) at 1/1000 dilution
Lane 1: Human fetal kidney tissue lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: U-87 MG cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Predicted band size: 38 kDa
Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified Anti-PPA2 antibody [EPR13086] ab180859 at a diltuion of 1/10.
All lanes: Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (Anti-PPA2 antibody [EPR13086] ab180859)
Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with unpurified Anti-PPA2 antibody [EPR13086] ab180859 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
This data was developed using Anti-PPA2 antibody [EPR13086] ab180859, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling PPA2 with unpurified Anti-PPA2 antibody [EPR13086] ab180859 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.