AB177935

Anti-PPA2 antibody [EPR13087(B)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2

3 Images

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Intracellular flow cytometric analysis of permeabilized 293 cellslabeling PPA2 with ab177935 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).

Supplier Data

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.

All lanes:

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935)

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

All lanes:

Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 38 kDa

false

Carrier free

Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13087(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes inorganic pyrophosphate (PubMed : 27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed : 27523598).

See full target information PPA2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 13: PubMed37999237

2023

PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie T Achleitner,Judith J M Jans,Laura Ebner,Johannes Spenger,Vassiliki Konstantopoulou,René G Feichtinger,Karin Brugger,Doris Mayr,Ron A Wevers,Christian Thiel,Saskia B Wortmann,Johannes A Mayr

Genetics in medicine : official journal of the American College of Medical Genetics 23:2415-2425 PubMed34400813

2021

PPA2-associated sudden cardiac death: extending the clinical and allelic spectrum in 20 new families.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Guimier,Melanie T Achleitner,Anne Moreau de Bellaing,Matthew Edwards,Loïc de Pontual,Kirti Mittal,Kyla E Dunn,Megan E Grove,Carolyn J Tysoe,Clémantine Dimartino,Jessie Cameron,Anil Kanthi,Anju Shukla,Florence van den Broek,Diptendu Chatterjee,Charlotte L Alston,Charlotte V Knowles,Laura Brett,Jan A Till,Tessa Homfray,Paul French,Georgia Spentzou,Noha A Elserafy,Kate S Lichkus,Bindu P Sankaran,Hannah L Kennedy,Peter M George,Alexa Kidd,Saskia B Wortmann,Dianna G Fisk,Tamara T Koopmann,Muhammad A Rafiq,Jason D Merker,Sumith Parikh,Priyanka Ahimaz,Robert G Weintraub,Alan S Ma,Christian Turner,Carolyn J Ellaway,Liza K Phillips,David R Thorburn,Wendy K Chung,Sajel L Kana,Ona M Faye-Petersen,Michelle L Thompson,Alexandre Janin,Karen McLeod,Ruth McGowan,Robert McFarland,Katta M Girisha,Deborah J Morris-Rosendahl,Anna C E Hurst,Claire L S Turner,Robert M Hamilton,Robert W Taylor,Fanny Bajolle,Christopher T Gordon,Jeanne Amiel,Johannes A Mayr,Kit Doudney

American journal of human genetics 99:674-682 PubMed27523597

2016

Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Kennedy,Tobias B Haack,Verity Hartill,Lavinija Mataković,E Regula Baumgartner,Howard Potter,Richard Mackay,Charlotte L Alston,Siobhan O'Sullivan,Robert McFarland,Grainne Connolly,Caroline Gannon,Richard King,Scott Mead,Ian Crozier,Wandy Chan,Chris M Florkowski,Martin Sage,Thomas Höfken,Bader Alhaddad,Laura S Kremer,Robert Kopajtich,René G Feichtinger,Wolfgang Sperl,Richard J Rodenburg,Jean Claude Minet,Angus Dobbie,Tim M Strom,Thomas Meitinger,Peter M George,Colin A Johnson,Robert W Taylor,Holger Prokisch,Kit Doudney,Johannes A Mayr

American journal of human genetics 99:666-673 PubMed27523598

2016

Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Guimier,Christopher T Gordon,François Godard,Gianina Ravenscroft,Myriam Oufadem,Christelle Vasnier,Caroline Rambaud,Patrick Nitschke,Christine Bole-Feysot,Cécile Masson,Stéphane Dauger,Cheryl Longman,Nigel G Laing,Béatrice Kugener,Damien Bonnet,Patrice Bouvagnet,Sylvie Di Filippo,Vincent Probst,Richard Redon,Philippe Charron,Agnès Rötig,Stanislas Lyonnet,Alain Dautant,Loïc de Pontual,Jean-Paul di Rago,Agnès Delahodde,Jeanne Amiel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPA2 antibody [EPR13087(B)]

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Western blot - Anti-PPA2 antibody [EPR13087(B)] (AB177935)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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