Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-PPA2 antibody [EPR13087(B)] (AB177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--western-blot-img102023.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPA2 antibody [EPR13087(B)] (AB177935)")
![Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (AB177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--immunoprecipitation-img64856.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (AB177935)")
![Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--flow-cytometry-intracellular-img28389.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (AB177935)")
Publications
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- Metabolites 13:2023PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie T Achleitner,Judith J M Jans,Laura Ebner,Johannes Spenger,Vassiliki Konstantopoulou,René G Feichtinger,Karin Brugger,Doris Mayr,Ron A Wevers,Christian Thiel,Saskia B Wortmann,Johannes A MayrPubMed 37999237
- Genetics in medicine : official journal of the American College of Medical Genetics 23:2415-24252021PPA2-associated sudden cardiac death: extending the clinical and allelic spectrum in 20 new families.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Guimier,Melanie T Achleitner,Anne Moreau de Bellaing,Matthew Edwards,Loïc de Pontual,Kirti Mittal,Kyla E Dunn,Megan E Grove,Carolyn J Tysoe,Clémantine Dimartino,Jessie Cameron,Anil Kanthi,Anju Shukla,Florence van den Broek,Diptendu Chatterjee,Charlotte L Alston,Charlotte V Knowles,Laura Brett,Jan A Till,Tessa Homfray,Paul French,Georgia Spentzou,Noha A Elserafy,Kate S Lichkus,Bindu P Sankaran,Hannah L Kennedy,Peter M George,Alexa Kidd,Saskia B Wortmann,Dianna G Fisk,Tamara T Koopmann,Muhammad A Rafiq,Jason D Merker,Sumith Parikh,Priyanka Ahimaz,Robert G Weintraub,Alan S Ma,Christian Turner,Carolyn J Ellaway,Liza K Phillips,David R Thorburn,Wendy K Chung,Sajel L Kana,Ona M Faye-Petersen,Michelle L Thompson,Alexandre Janin,Karen McLeod,Ruth McGowan,Robert McFarland,Katta M Girisha,Deborah J Morris-Rosendahl,Anna C E Hurst,Claire L S Turner,Robert M Hamilton,Robert W Taylor,Fanny Bajolle,Christopher T Gordon,Jeanne Amiel,Johannes A Mayr,Kit DoudneyPubMed 34400813
- American journal of human genetics 99:674-6822016Sudden Cardiac Death Due to Deficiency of the Mitochondrial Inorganic Pyrophosphatase PPA2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah Kennedy,Tobias B Haack,Verity Hartill,Lavinija Mataković,E Regula Baumgartner,Howard Potter,Richard Mackay,Charlotte L Alston,Siobhan O'Sullivan,Robert McFarland,Grainne Connolly,Caroline Gannon,Richard King,Scott Mead,Ian Crozier,Wandy Chan,Chris M Florkowski,Martin Sage,Thomas Höfken,Bader Alhaddad,Laura S Kremer,Robert Kopajtich,René G Feichtinger,Wolfgang Sperl,Richard J Rodenburg,Jean Claude Minet,Angus Dobbie,Tim M Strom,Thomas Meitinger,Peter M George,Colin A Johnson,Robert W Taylor,Holger Prokisch,Kit Doudney,Johannes A MayrPubMed 27523597
- American journal of human genetics 99:666-6732016Biallelic PPA2 Mutations Cause Sudden Unexpected Cardiac Arrest in Infancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Guimier et. al.PubMed 27523598
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Hydrolyzes inorganic pyrophosphate (PubMed:27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed:27523598).
Alternative names
HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2
Recommended products
Rabbit Recombinant Monoclonal PPA2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13087(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--western-blot-img102023.jpg "Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935)")
Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935)
All lanes: Western blot - Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: Human fetal kidney tissue lysate at 10 µg
Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg
Lane 5: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Predicted band size: 38 kDa
![Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--immunoprecipitation-img64856.jpg "Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935)")
Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935)
Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.
All lanes: Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (ab177935)
Predicted band size: 38 kDa
![Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (ab177935), expandable thumbnail](https://content.abcam.com/products/images/ppa2-antibody-epr13087b-ab177935--flow-cytometry-intracellular-img28389.jpg "Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (ab177935)")
Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] (ab177935)
Intracellular flow cytometric analysis of permeabilized 293 cellslabeling PPA2 with ab177935 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
Downloads
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