JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250010

Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PPA2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HSPC124, PPA2, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2

3 Images
Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)

This data was developed using ab177935, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells labeling PPA2 with ab177935 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)
  • IP

Supplier Data

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)

This data was developed using ab177935, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.

All lanes:

Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] (<a href='/en-us/products/primary-antibodies/ppa2-antibody-epr13087b-ab177935'>ab177935</a>)

Predicted band size: 38 kDa

false

Western blot - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)
  • WB

Supplier Data

Western blot - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (AB250010)

This data was developed using ab177935, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPA2 antibody [EPR13087(B)] (<a href='/en-us/products/primary-antibodies/ppa2-antibody-epr13087b-ab177935'>ab177935</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13087(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB32141

Anti-PP2A alpha + beta antibody [Y119]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (AB32141)

  • 4
  • 5
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
Primary Antibodies

AB175223

Anti-Pyrophosphatase 1 antibody [EPR11281]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Pyrophosphatase 1 antibody [EPR11281] (AB175223)

  • 0
  • 0
Primary Antibodies

AB133579

Anti-PSMA antibody [EPR6253]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

  • 3
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250010 is the carrier-free version of ab177935.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Hydrolyzes inorganic pyrophosphate (PubMed : 27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed : 27523598).
See full target information PPA2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com