Anti-PPAR alpha antibody (ab126285) is a rabbit polyclonal antibody detecting PPAR alpha in Western Blot. Suitable for Human, Mouse.
- Over 20 publications
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- Journal of dairy science 107:3127-31392023Inositol-requiring enzyme 1α and c-Jun N-terminal kinase axis activation contributes to intracellular lipid accumulation in calf hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Gao,Yanxi Wang,Siyu Liu,Guojin Li,Qi Shao,Cai Zhang,Liguang Cao,Kai Liu,Wenrui Gao,Zifeng Yang,Yifei Dong,Xiliang Du,Lin Lei,Guowen Liu,Xinwei LiPubMed 37939835
- Nature communications 14:54512023Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhong,Xiaofang He,Xinxin Gao,Qiaohong Liu,Yu Zhao,Ying Hong,Weize Zhu,Juan Yan,Yifan Li,Yan Li,Ningning Zheng,Yiyang Bao,Hao Wang,Junli Ma,Wenjin Huang,Zekun Liu,Yuanzhi Lyu,Xisong Ke,Wei Jia,Cen Xie,Yiyang Hu,Lili Sheng,Houkai LiPubMed 37673856
- Frontiers in nutrition 9:10697972022Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengnan Zhu,Weijia Xu,Jun Liu,Feng Guan,Aichun Xu,Jin Zhao,Jian GePubMed 36579075
- Nutrients 14:2022Ramulus Mori (Sangzhi) Alkaloids Ameliorate Obesity-Linked Adipose Tissue Metabolism and Inflammation in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Wen Sun,Chun-Fang Lian,Yan-Min Chen,Jun Ye,Wei Chen,Yue Gao,Hong-Liang Wang,Li-Li Gao,Yu-Ling Liu,Yan-Fang YangPubMed 36501080
- Frontiers in immunology 13:10054302022The protective role of caffeic acid on bovine mammary epithelial cells and the inhibition of growth and biofilm formation of Gram-negative bacteria isolated from clinical mastitis milk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianle Xu,Hao Zhu,Run Liu,Xinyue Wu,Guangjun Chang,Yi Yang,Zhangping YangPubMed 36341408
- Frontiers in pharmacology 13:9155352022Systematic characterization of Puerariae Flos metabolites and assessment of its protective mechanisms against alcoholic liver injury in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin Qu,Qiuyue Chen,Tianfu Wei,Ning Dou,Dong Shang,Dan YuanPubMed 36110520
- Frontiers in pharmacology 13:9155922022Bailing capsule () ameliorates renal triglyceride accumulation through the PPARα pathway in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Zhang,Xinhua Xiao,Ming Li,Miao Yu,Fan PingPubMed 36091833
- Journal of applied oral science : revista FOB 30:e202200762022In vitro and in vivo study of the pathogenic role of PPARα in experimental periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Zheqing Jiang,Ana Keohane,Yang HuPubMed 35830121
- Frontiers in bioengineering and biotechnology 10:8585582022LncRNA MALAT1 Promotes PPARα/CD36-Mediated Hepatic Lipogenesis in Nonalcoholic Fatty Liver Disease by Modulating miR-206/ARNT Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Xiang,Yuan-Yuan Deng,Hui-Xia Liu,Ying PuPubMed 35769097
- Antioxidants (Basel, Switzerland) 11:2022(Sangzhi) Alkaloids Alleviate High-Fat Diet-Induced Obesity and Nonalcoholic Fatty Liver Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Min Chen,Chun-Fang Lian,Qian-Wen Sun,Ting-Ting Wang,Yuan-Yuan Liu,Jun Ye,Li-Li Gao,Yan-Fang Yang,Shuai-Nan Liu,Zhu-Fang Shen,Yu-Ling LiuPubMed 35624769
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Macaque monkey | Predicted |
| Orangutan | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Dog, Macaque monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Alternative names
NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1
Recommended products
Anti-PPAR alpha antibody (ab126285) is a rabbit polyclonal antibody detecting PPAR alpha in Western Blot. Suitable for Human, Mouse.
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PPAR alpha antibody (ab126285) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of PPAR alpha?
Anti-PPAR alpha (ab126285) specifically detects a band for PPAR alpha (UniProt: Q07869) at a molecular weight of 52kDa.
Trusted by the scientific community
Anti-PPAR alpha (ab126285) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-PPAR alpha antibody (ab126285)
All lanes: Western blot - Anti-PPAR alpha antibody (ab126285) at 1 µg/mL
Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 37 kDa, 50 kDa, 52 kDa
Exposure time: 1min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-PPAR alpha antibody (ab126285)
PPAR alpha western blot using anti-PPAR alpha antibody ab126285. Publication image and figure legend from Shao, Z., Koh, W., et al., 2020, Sci Rep, PubMed 32313136.
ab126285 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126285 please see the product overview.
Validation of One-month-old Mouse Heart RNA-Sequencing Results by qRT-PCR and Western Blot. Samples (RNAs and proteins) being analyzed were extracted from WT and Lmna−/− mouse heart tissues at 1 month of age. (a) RT-qPCR validation of down-regulated genes (ACDVL, ATP5G1, CRAT, ECH1, KCDN2, P2RY1, PPARA) identified by RNA-Sequencing. The same ∆∆Ct method was used for analysis as described in Fig. 3 legends. The relative expression levels of all the listed genes are showed as “mean ± SEM” on Y axis. The expression levels of all the listed genes among 1-month Lmna−/− mouse hearts were significantly lower than the levels among 1-month WT mouse hearts (p < 0.05). (b) RT-qPCR validation of up-regulated genes (DUSP4, LOX, FHL1, MYOM2, NMRK2) identified by RNA-Sequencing. The same ∆∆Ct method was used for analysis. The relative expression levels of all the listed genes are showed as “mean ± SEM” on Y axis. The expression levels in 1-month Lmna−/− mouse hearts were significantly higher than those in 1-month WT mouse hearts for all the genes (p < 0.05). (c) Western blot validation of protein expressions of representative down-regulated (ECH1 and PPARA) and up-regulated (DUSP4 and FHL1) genes. β-actin (for ECH1 and PPARA) or GAPDH (for DUSP4 and FHL1) was used as a loading control. Three repeated experiments were conducted for each protein with similar results showed in the figure.
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