AB215270

Anti-PPAR alpha antibody

Name: Anti-PPAR alpha antibody (ab215270) | Abcam
Brand: Abcam Limited
SKU: AB215270
Price: 485 USD
Availability: InStock

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(23 Publications)

Rabbit Polyclonal PPAR alpha antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human PPARA aa 300-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

2 Images

Flow Cytometry (Intracellular) - Anti-PPAR alpha antibody (AB215270)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPAR alpha antibody (AB215270)

Intracellular Flow Cytometry of A431 cells with ab215439 at 1/50

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB215270)

All lanes:

Western blot - Anti-PPAR alpha antibody (ab215270) at 1/300 dilution

Lane 1:

Mouse heart lysate

Lane 2:

Mouse liver lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human PPARA aa 300-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q07869

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

See full target information PPARA

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Biological procedures online 27:14 PubMed40229695

2025

Differences in Fatty Acid Metabolism between MCDD and HFD Induced Metabolic Dysfunction-associated Fatty Liver Disease Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Xuan Wang,Xin-Zhu Liu,Zhen Guo,Hui-Lin Zhang,Li Qi,Jia Liu,Ping Liu,Guo-Xiang Xie,Xiao-Ning Wang

Endocrine journal 72:79-91 PubMed39443113

2024

Inhibition of mmu_circ_0009303 improves metabolic dysfunction-associated steatotic liver disease by regulating lipid metabolism and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Zhou,Wu Li,Xiaowei Chi,Dingchun Li,Chunxia Yang,Zhiwen Duan

Scientific reports 14:23816 PubMed39394435

2024

Selective activation of PPARα by pemafibrate mitigates peritoneal inflammation and fibrosis through suppression of NLRP3 inflammasome and modulation of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Shinkai,Kensuke Sasaki,Ryo Tamura,Takeshi Ike,Akira Takahashi,Yosuke Osaki,Naoki Ishiuchi,Yujiro Maeoka,Ayumu Nakashima,Takao Masaki

Research (Washington, D.C.) 7:0400 PubMed38939042

2024

Brain Short-Chain Fatty Acids Induce ACSS2 to Ameliorate Depressive-Like Behavior via PPARγ-TPH2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Chen,Xinyi Xu,Yaxin Guo,Ming Zhao,Yubin Li,Tian Zhou,Xinyue Zhang,Jie Gao,Faliang Zhu,Chun Guo,Yongyu Shi,Qun Wang,Wenxian Wu,Lining Zhang,Yan Li

Chinese medicine 19:76 PubMed38831430

2024

Chrysanthemum morifolium Ramat extract and probiotics combination ameliorates metabolic disorders through regulating gut microbiota and PPARα subcellular localization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Gao,Zhigang Zhu,Yiyang Bao,Yifan Li,Weize Zhu,Xiaofang He,Xinyu Ge,Wenjin Huang,Hao Wang,Wenjing Wei,Jun Du,Liang Chen,Houkai Li,Lili Sheng

Cell death discovery 10:164 PubMed38575595

2024

Hypoxic preconditioning accelerates the healing of ischemic intestinal injury by activating HIF-1α/PPARα pathway-mediated fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Linxia Li,Yanqi Liu,Na Zhi,Yaoxuan Ji,Jialing Xu,Guoyun Mao,Yazhou Wang,Jin Ma,Yunying Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2304702 PubMed38145969

2023

Induction of Fatty Acid Oxidation Underlies DNA Damage-Induced Cell Death and Ameliorates Obesity-Driven Chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Sunsook Hwang,Seungyeon Yang,Kyungsoo Park,Byungjoo Kim,Minjoong Kim,Seungmin Shin,Ahyoung Yoo,Jiyun Ahn,Juneil Jang,Yeong Shin Yim,Rho H Seong,Seung Min Jeong

Nature communications 14:5451 PubMed37673856

2023

Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhong,Xiaofang He,Xinxin Gao,Qiaohong Liu,Yu Zhao,Ying Hong,Weize Zhu,Juan Yan,Yifan Li,Yan Li,Ningning Zheng,Yiyang Bao,Hao Wang,Junli Ma,Wenjin Huang,Zekun Liu,Yuanzhi Lyu,Xisong Ke,Wei Jia,Cen Xie,Yiyang Hu,Lili Sheng,Houkai Li

Frontiers in pharmacology 13:876937 PubMed35865947

2022

AKT/PACS2 Participates in Renal Vascular Hyperpermeability by Regulating Endothelial Fatty Acid Oxidation in Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Shu,Shuhua Chen,Hong Xiang,Ruoru Wu,Xuewen Wang,Jie Ouyang,Jing Zhang,Huiqin Liu,Alex F Chen,Hongwei Lu

Frontiers in medicine 8:788663 PubMed35004756

2021

Peroxisome Proliferator-Activated Receptor α Activation Protects Retinal Ganglion Cells in Ischemia-Reperfusion Retinas.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yao,Xuan Zhang,Xueyan Yao,Xiaohua Ren,Xiaobo Xia,Jian Jiang,Lexi Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPAR alpha antibody

Flow Cytometry (Intracellular) - Anti-PPAR alpha antibody (AB215270)

Western blot - Anti-PPAR alpha antibody (AB215270)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (23)

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