AB227074

Anti-PPAR alpha antibody

Name: Anti-PPAR alpha antibody (ab227074) | Abcam
Brand: Abcam Limited
SKU: AB227074
Price: 410 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(29 Publications)

Anti-PPAR alpha antibody (ab227074) is a rabbit polyclonal antibody detecting PPAR alpha in Western Blot. Suitable for Human, Rat.

- Over 10 publications

View Alternative Names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

3 Images

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/1000 dilution

Lane 1:

Non-transfected (-) unboiled HepG2 whole cell extracts at 30 µg

Lane 2:

Transfected (+) unboiled HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/500 dilution

All lanes:

HeLa Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/2000 dilution

Lane 1:

Rat kidney tissue extracts at 50 µg

Lane 2:

Rat liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human PPARA. The exact immunogen used to generate this antibody is proprietary information.

Q07869

Specificity

This antibody does not cross-react with human PPAR gamma or PPAR delta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PPAR alpha antibody (ab227074) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Rat samples.

What is the molecular weight of PPAR alpha?
Anti-PPAR alpha (ab227074) specifically detects a band for PPAR alpha (UniProt: Q07869) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-PPAR alpha (ab227074) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

See full target information PPARA

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5411-5427 PubMed40959276

2025

Therapeutic Activation of PPARα Inhibits Transformed Follicular Lymphoma Tumorigenesis via the FOXM1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Qin,Hui Zhou,Jie Zhou,Chendi Xie,Shuman Jia,Xingxing Yu,Yan Hong,Li Zhang,Yueting Huang,Yong Zhou,Bing Xu,Jie Zha

PPAR research 2024:4164906 PubMed39735727

2024

Clinical Relevance and Drug Modulation of PPAR Signaling Pathway in Triple-Negative Breast Cancer: A Comprehensive Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Zhang,Yunduo Liu,Mei Zhang,Guanjie Li,Siling Zhu,Keping Xie,Bin Xiao,Linhai Li

Life science alliance 8: PubMed39578076

2024

SPHK1/S1PR1/PPAR-α axis restores TJs between uroepithelium providing new ideas for IC/BPS treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Zhang,Qingyu Ge,Tianpeng Du,Yuhao Kuang,Zongyao Fan,Xinyi Jia,Wenjin Gu,Zhengsen Chen,Zhongqing Wei,Baixin Shen

Cardiovascular diagnosis and therapy 14:832-847 PubMed39513140

2024

The transcription factor PPARA mediates SIRT1 regulation of NCOR1 to protect damaged heart cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Fang Zhou,Yuntao Luo,Xu Deng,Xinyu Chen,Qin Yi

Acta pharmaceutica Sinica. B 14:3949-3963 PubMed39309511

2024

Schisanhenol ameliorates non-alcoholic fatty liver disease inhibiting miR-802 activation of AMPK-mediated modulation of hepatic lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Qi Xiao,Hongmei Zhao,Jianuo Zhang,Chunyan Yang,Yucen Zou,Bengang Zhang,Jiushi Liu,Haitao Sun,Haitao Liu

Rheumatology (Oxford, England) 63:2578-2589 PubMed38837706

2024

FTY720 ameliorates experimental MPO-ANCA-associated vasculitis by regulating fatty acid oxidation via the neutrophil PPARα-CPT1a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Xue Wang,Luo-Yi Wang,Xiang-Yu Han,Su-Fang Chen,Xiao-Jing Sun,Zhi-Ying Li,Mark A Little,Ming-Hui Zhao,Min Chen

Journal of pharmaceutical analysis 14:100911 PubMed38807706

2024

Alginate oligosaccharide-mediated butyrate-HIF-1α axis improves skin aging in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gao,Yixuan Li,Xiaoyu Wang,Fazheng Ren

Journal of orthopaedic surgery and research 19:109 PubMed38308345

2024

PDK4 inhibits osteoarthritis progression by activating the PPAR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengnan Li,Lifeng Xie,Hui Zeng,Yaohong Wu

Reproductive sciences (Thousand Oaks, Calif.) 31:1573-1585 PubMed38177949

2024

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Weijian Zeng,Yanyan Hou,Wei Gu,Zheng Chen

Cells 12: PubMed37566072

2023

RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wu,Xiaobei Wang,Min Zhang,Parker Mathews,Yubin Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPAR alpha antibody

Western blot - Anti-PPAR alpha antibody (AB227074)

Western blot - Anti-PPAR alpha antibody (AB227074)

Western blot - Anti-PPAR alpha antibody (AB227074)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (29)

Product promise