AB245119

Anti-PPAR alpha antibody [EPR21244]

BOND RX™ Validated
20ul selling size
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal PPAR alpha antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (AB245119)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (AB245119)

IHC image of PPAR alpha staining in a section of formalin-fixed paraffin-embedded normal human duodenum*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab245119 at 0.03μg/ml concentration, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21244

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.03 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p>In-house testing found this antibody did not recognise PPARA in western blot.</p>" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/Rabbit Fc).

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

See full target information PPARA

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70464 PubMed40985995

2025

CD103CD8 tissue-resident memory T lymphocytes of melanoma boost anti-tumour immunity and predict immunotherapy outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Junquan Song,Yinlam Li,Kangjie Shen,Jiangying Xuan,Yuan Gao,Lili Lu,Zhi Pang,Lu Wang,Yang Yang,Zixu Gao,Qianrong Hu,Yu Zhu,Chenlu Wei,Shaoluan Zheng,Rongkui Luo,Yingyong Hou,Yuhong Zhou,Chuanyuan Wei,Jianying Gu

Pathophysiology : the official journal of the International Society for Pathophysiology 32: PubMed40265441

2025

Hepatoprotective and Antiatherosclerotic Effects of Oleoylethanolamide-Based Dietary Supplement in Dietary-Induced Obesity in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Darya Ivashkevich,Arina Ponomarenko,Igor Manzhulo,Anastasia Egoraeva,Inessa Dyuizen

Chemistry & biodiversity 21:e202400564 PubMed38708558

2024

Chlorogenic Acid and its Isomers Attenuate NAFLD by Mitigating Lipid Accumulation in Oleic Acid-Induced HepG2 Cells and High-Fat Diet- Fed Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Tie,Jin Ding,Yidan Gao,Honglun Wang

Journal of assisted reproduction and genetics 40:1447-1459 PubMed37204637

2023

miR-4433a-3p promotes granulosa cell apoptosis by targeting peroxisome proliferator-activated receptor alpha and inducing immune cell infiltration in polycystic ovarian syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Xi Yao,Ying Mo,Ming-Wei Chen,Si-Chen Li,Jian-Qiao Liu,Hai-Ying Liu

Journal of nanobiotechnology 20:3 PubMed34983531

2022

Ceria nanoparticles ameliorate renal fibrosis by modulating the balance between oxidative phosphorylation and aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengling Wang,Feng Zeng,Fengling Ning,Yinhang Wang,Shilin Zhou,Jiaqi He,Cong Li,Cong Wang,Xiaolin Sun,Dongliang Zhang,Jisheng Xiao,Ping Hu,Svetlana Reilly,Hong Xin,Xudong Xu,Xuemei Zhang

Frontiers in pharmacology 12:719532 PubMed34630097

2021

Proteomic Analysis Reveals the Protective Effects of Yiqi Fumai Lyophilized Injection on Chronic Heart Failure by Improving Myocardial Energy Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Han,Yi Zhang,Ou Qiao,Haixia Ji,Xinyu Zhang,Wenzhe Wang,Xia Li,Juan Wang,Dekun Li,Aichun Ju,Changxiao Liu,Wenyuan Gao

Molecular medicine reports 24: PubMed33982779

2021

polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Yuan Tang,Zhenyan Song,Jinwen Ge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPAR alpha antibody [EPR21244]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (AB245119)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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