Anti-PPAR alpha (phospho S12) antibody

• WB

Supplier Data

Western blot - Anti-PPAR alpha (phospho S12) antibody (AB3484)

All lanes:

Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/1000 dilution

Lane 1:

U-87 MG at 20 µg

Lane 2:

MCF7 at 20 µg

Lane 3:

MDA-MB-231 at 20 µg

Lane 4:

C2C12 at 20 µg

Lane 5:

Hep G2 at 20 µg

Lane 6:

NIH/3T3 at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) at 1/2500 dilution

Predicted band size: 52 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (AB3484)

Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1 : 200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• ICC/IF

AbReview19711****

Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (AB3484)

ab3484 staining PPAR alpha (phospho S12) in Mouse neuronal cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. A Cy2^®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (AB3484)

Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1 : 200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (AB3484)

Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of 3T3-L1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1 : 200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• WB

Supplier Data

Western blot - Anti-PPAR alpha (phospho S12) antibody (AB3484)

All lanes:

Western blot - Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/200 dilution

Lane 1:

C2C12 cell lysate at 25 µg

Lane 2:

NIH-3T3 cell lysate at 25 µg

Lane 3:

3T3-L1 cell lysate at 25 µg

Lane 4:

HepG2 cell lysate at 25 µg

Predicted band size: 52 kDa

Observed band size: 52 kDa

false