Rabbit Recombinant Monoclonal PPAR delta antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img28722.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)")
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img55231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)")
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img22734.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)")
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img147612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)")
Publications
Filter by
- Journal of gastroenterology and hepatology 38:2215-22272023Mdivi1 ameliorates mitochondrial dysfunction in non-alcoholic steatohepatitis by inhibiting JNK/MFF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Quan,Diwen Shou,Siqi Yang,Jiemin Cheng,Yongqiang Li,Chen Huang,Huiting Chen,Yongjian ZhouPubMed 37839851
- Journal of virology 95:2021Fowl Adenovirus Serotype 4 Induces Hepatic Steatosis via Activation of Liver X Receptor-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Yuan,Lei Hou,Li Wei,Rong Quan,Jing Wang,Hao Liu,Jue LiuPubMed 33361420
- Chemosphere 236:1242642019Dichotomy of bisphenol A-induced expression of peroxisome proliferator-activated receptors in hepatic and testicular tissues in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShikha Sharma et. al.PubMed 31319301
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Recombinant full length protein - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/2000 | Notes - |
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Additional Targets
PPARA
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
Recommended products
Rabbit Recombinant Monoclonal PPAR delta antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR16673(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img28722.jpg "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)")
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.
All lanes: Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution
Lane 1: Human PPAR alpha full length recombinant protein at 0.01 µg
Lane 2: Human PPAR beta full length recombinant protein at 0.01 µg
Lane 3: Human PPAR gamma fragment recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa, 52 kDa
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img55231.jpg "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)")
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: Human fetal heart tissue lysate at 10 µg
Lane 3: Human fetal kidney tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 50 kDa, 52 kDa
Observed band size: 50 kDa
Exposure time: 2s
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img22734.jpg "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)")
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4 and 5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.
All lanes: Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1: Human skeletal muscle lysate at 20 µg
Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 5: Mouse placenta lysate at 20 µg
Lane 6: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 7: Rat placenta lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa, 52 kDa
Observed band size: 50 kDa
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865), expandable thumbnail](https://content.abcam.com/products/images/ppar-alpha-ppar-beta-delta-antibody-epr166732-ab178865--western-blot-img147612.jpg "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)")
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3 and 4: 2 seconds; Lane 5,6,7,8,9,10 and 11: 1 second.
All lanes: Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Lane 5: Rat brain lysate at 10 µg
Lane 6: Rat heart lysate at 10 µg
Lane 7: Rat kidney lysate at 10 µg
Lane 8: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 9: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 10: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 11: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa, 52 kDa
Observed band size: 50 kDa
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Product protocols
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