Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)]

• WB

Supplier Data

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,3,4 and 5 : 2 seconds; Lane 6 : 1 second; Lane 7 : 30 seconds.

All lanes:

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 5:

Mouse placenta lysate at 20 µg

Lane 6:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 7:

Rat placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa,52 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 1 second; Lane 3 : 3 minutes.

Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.

All lanes:

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution

Lane 1:

Human PPAR alpha full length recombinant protein at 0.01 µg

Lane 2:

Human PPAR beta full length recombinant protein at 0.01 µg

Lane 3:

Human PPAR gamma fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa,52 kDa

false

• WB

Supplier Data

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 50 kDa,52 kDa

Observed band size: 50 kDa

false

Exposure time: 2s

• WB

Supplier Data

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (AB178865)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,3 and 4 : 2 seconds; Lane 5,6,7,8,9,10 and 11 : 1 second.

All lanes:

Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat kidney lysate at 10 µg

Lane 8:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 9:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 10:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 11:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa,52 kDa

Observed band size: 50 kDa

false