Rabbit Polyclonal PPAR delta antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human PPARD aa 1-100.
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Publications
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- BMC cancer 23:10422023Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinghui Jia,Liting Liao,Yongshuai Jiang,Xiangyu Hu,Guotao Lu,Weiming Xiao,Weijuan Gong,Xiaoqin JiaPubMed 37904094
- International journal of molecular sciences 24:2023Smad3 Mediates Diabetic Dyslipidemia and Fatty Liver in db/db Mice by Targeting PPARδ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuijun He,Yu Zhong,Honglian Wang,Patrick Ming-Kuen Tang,Vivian Weiwen Xue,Xiaocui Chen,Jiaoyi Chen,Xiaoru Huang,Cheng Wang,Huiyao LanPubMed 37511155
- Nature communications 13:67232022CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhillip M Brailey,Lauren Evans,Juan Carlos López-Rodríguez,Anthony Sinadinos,Victoria Tyrrel,Gavin Kelly,Valerie O'Donnell,Peter Ghazal,Susan John,Patricia BarralPubMed 36344546
- iScience 25:1041842022Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeroy C Joseph,Jianting Shi,Quynh N Nguyen,Victoria Pensiero,Chris Goulbourne,Robert C Bauer,Hanrui Zhang,John P MorrowPubMed 35494220
- eNeuro 9:2022Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnddre Osmar Valdivia,Sanjoy K BhattacharyaPubMed 35027445
- Biology 10:2021PPARα, δ and FOXO1 Gene Silencing Overturns Palmitate-Induced Inhibition of Pyruvate Oxidation Differentially in C2C12 Myotubes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHung-Che Chien,Despina Constantin,Paul L Greenhaff,Dumitru Constantin-TeodosiuPubMed 34827089
- Histology and histopathology 36:547-5582021Defective expression of the peroxisome regulators PPARα receptors and lysogenesis with increased cellular senescence in the venous wall of chronic venous disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A Ortega,Oscar Fraile-Martínez,Leonel Pekarek,Miguel A Alvarez-Mon,Ángel Asúnsolo,Lara Sanchez-Trujillo,Santiago Coca,Julia Buján,Melchor Álvarez-Mon,Natalio García-Honduvilla,Felipe SainzPubMed 33645625
- Experimental and therapeutic medicine 21:4382021Laminar shear stress upregulates the expression of PPARs in vascular endothelial cells under high free fatty acid-induced stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Lin Wang,Chen-Te Chen,Che-Se Tung,Min-Chien TsaiPubMed 33747175
- Stem cell research & therapy 11:4442020DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangcao Jin et. al.PubMed 33076979
- Behavioural neurology 2020:10805212020Wild Bitter Melon Exerts Anti-Inflammatory Effects by Upregulating Injury-Attenuated CISD2 Expression following Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoon-Man Kung et. al.PubMed 33062068
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPARD aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q03181
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa. |
Species Human | Dilution info 2 µg/mL | Notes Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
Recommended products
Rabbit Polyclonal PPAR delta antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human PPARD aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPARD aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q03181
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-PPAR delta antibody (ab23673)
All lanes: Western blot - Anti-PPAR delta antibody (ab23673) at 1/1000 dilution
All lanes: Rat brain tissue lysate at 100 µg
SecondaryAll lanes: HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa
Exposure time: 30s
Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab23673)
Immunofluorescent staining of HT-29 cells (HT-29 whole cell lysate ab3952) (nuclear staining) using ab23673.
Western blot - Anti-PPAR delta antibody (ab23673)
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta
(~40kDa) is also detected in certain murine tissues.All lanes: Western blot - Anti-PPAR delta antibody (ab23673)
Lane 1: Human cerebral cortex (30ug)
Lane 2: Murine liver (30ug)
Predicted band size: 50 kDa
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