Anti-PPAR delta antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (AB8937)

ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

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Western blot - Anti-PPAR delta antibody (AB8937)

Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysate

Lane 1 : ab8937 1/500
Lane 2 : ab8937 1/1000

Secondary antibody : Goat anti-Rabbit (HRP) (ab6721) at 1/2000

Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1 : ab8937 1/500 Lane 2 : ab8937 1/1000 Secondary antibody : Goat anti-Rabbit (HRP) (ab6721) at 1/2000

All lanes:

Western blot - Anti-PPAR delta antibody (ab8937)

Predicted band size: 50 kDa

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• WB

CiteAb

Western blot - Anti-PPAR delta antibody (AB8937)

Western Blotting using Anti-PPAR delta antibody, ab8937. Publication image from Zheng, X. et al., 2022, Nat Commun, 35562376. Legend direct from paper.

PPARδ expression is upregulated in human and mouse PanINs and regulated by KRASmu activity.a, b PPARδ protein expression in human normal, PanIN 1-2, and PDAC pancreatic tissues in a tissue microarray (TMA), measured by immunohistochemistry (IHC). a Representative images of PPARδ IHC staining of the indicated groups of human pancreatic tissue samples. b PPARδ IHC scores of normal (n = 11), PanIN 1-2 (n = 7), and PDAC (n = 6) pancreatic tissues obtained from TMA. c, d PPARδ mRNA expression levels in human normal and paired PanIN 1-2 (n = 10), measured by RNAscope in situ hybridization. c Representative images of PPARδ RNAscope in situ hybridization staining of the slides. Positive PPARδ mRNA staining is shown in red dots. d Quantitative results of PPARδ intensity scores. e Representative images of hematoxylin and eosin (H&E) staining, PPARδ RNAscope in situ hybridization, phospho-Erk1/2 (p-Erk1/2) IHC staining, Alcian blue staining, and Sirius red staining of normal, PanIN 1-2, and PanIN 3 pancreatic tissues in KC mice. f Quantitative data of PPARδ mRNA intensity scores of normal, PanIN 1-2, and PanIN 3 pancreatic tissues in KC mice as described in panel e (n = 10 biologically independent samples). g, h Two independent iKPC cell lines were treated with 1 µg/ml doxycycline (DOX-on) or control solvent (DOX-off) for 72 h, and then PPARδ mRNA expression from three independent experiments was measured by qRT-PCR (g), and PPARδ and p-Erk1/2 protein levels were measured by Western blot (h). i, j Two iKPC mouse PDAC cell lines cultured with 1 µg/ml DOX were treated with 1 µM MEK inhibitor PD0325901 for 24 h, and then PPARδ mRNA expression from three independent experiments was measured by qRT-PCR (i), and PPARδ and p-Erk1/2 protein levels were measured by Western blot (j). Data are mean ± SEM. For (b, f), one-way ANOVA with Bonferroni correction, and for (d), (g, i), unpaired two-tailed Student’s t-test. *P < .05 and ****P < .0001. Source data are provided in a Source Data file.

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• WB

CiteAb

Western blot - Anti-PPAR delta antibody (AB8937)

Western Blotting using Anti-PPAR delta antibody, ab8937. Publication image from Zheng, X. et al., 2022, Nat Commun, 35562376. Legend direct from paper.

PPARδ expression is upregulated in human and mouse PanINs and regulated by KRASmu activity.a, b PPARδ protein expression in human normal, PanIN 1-2, and PDAC pancreatic tissues in a tissue microarray (TMA), measured by immunohistochemistry (IHC). a Representative images of PPARδ IHC staining of the indicated groups of human pancreatic tissue samples. b PPARδ IHC scores of normal (n = 11), PanIN 1-2 (n = 7), and PDAC (n = 6) pancreatic tissues obtained from TMA. c, d PPARδ mRNA expression levels in human normal and paired PanIN 1-2 (n = 10), measured by RNAscope in situ hybridization. c Representative images of PPARδ RNAscope in situ hybridization staining of the slides. Positive PPARδ mRNA staining is shown in red dots. d Quantitative results of PPARδ intensity scores. e Representative images of hematoxylin and eosin (H&E) staining, PPARδ RNAscope in situ hybridization, phospho-Erk1/2 (p-Erk1/2) IHC staining, Alcian blue staining, and Sirius red staining of normal, PanIN 1-2, and PanIN 3 pancreatic tissues in KC mice. f Quantitative data of PPARδ mRNA intensity scores of normal, PanIN 1-2, and PanIN 3 pancreatic tissues in KC mice as described in panel e (n = 10 biologically independent samples). g, h Two independent iKPC cell lines were treated with 1 µg/ml doxycycline (DOX-on) or control solvent (DOX-off) for 72 h, and then PPARδ mRNA expression from three independent experiments was measured by qRT-PCR (g), and PPARδ and p-Erk1/2 protein levels were measured by Western blot (h). i, j Two iKPC mouse PDAC cell lines cultured with 1 µg/ml DOX were treated with 1 µM MEK inhibitor PD0325901 for 24 h, and then PPARδ mRNA expression from three independent experiments was measured by qRT-PCR (i), and PPARδ and p-Erk1/2 protein levels were measured by Western blot (j). Data are mean ± SEM. For (b, f), one-way ANOVA with Bonferroni correction, and for (d), (g, i), unpaired two-tailed Student’s t-test. *P < .05 and ****P < .0001. Source data are provided in a Source Data file.

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