Rabbit Polyclonal PPAR delta antibody. Suitable for WB, ICC/IF, ELISA, IHC-P and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ppard aa 1-50.
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- Metabolites 12:2022PPARβ/δ Augments IL-1β-Induced COX-2 Expression and PGE2 Biosynthesis in Human Mesangial Cells via the Activation of SIRT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqing Li,Rong Cao,Tingting Gu,Cong Cao,Tingyue Chen,Youfei Guan,Xiaoyan ZhangPubMed 35888719
- Nature communications 13:26652022Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Liu,Yasunori Deguchi,Daoyan Wei,Fuyao Liu,Micheline J Moussalli,Eriko Deguchi,Donghui Li,Huamin Wang,Lovie Ann Valentin,Jennifer K Colby,Jing Wang,Xiaofeng Zheng,Haoqiang Ying,Mihai Gagea,Baoan Ji,Jiaqi Shi,James C Yao,Xiangsheng Zuo,Imad ShureiqiPubMed 35562376
- PLoS neglected tropical diseases 14:e00088502020The function of peroxisome proliferator-activated receptors PPAR-γ and PPAR-δ in Mycobacterium leprae-induced foam cell formation in host macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqian Luo et. al.PubMed 33075048
- International journal of molecular sciences 21:2020Investigating the Role of PPARβ/δ in Retinal Vascular Remodeling Using /-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSze Yuan Ho et. al.PubMed 32575793
- Scientific reports 8:91012018WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShintaro Mandai et. al.PubMed 29904119
- Cellular physiology and biochemistry : internation 47:545-5552018MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXumin Hu et. al.PubMed 29794473
- Cell transplantation 26:1405-14172017PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial Fluid.Applications:WBReactive species:HumanBruce E Heck,Joshua J Park,Vishruti Makani,Eun-Cheol Kim,Dong Hyun KimPubMed 28901183
- PloS one 12:e01801432017Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia.
- PloS one 12:e01730352017Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells.
- Nature chemical biology 12:1075-10832016Identification and characterization of PPARα ligands in the hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvik Roy et. al.PubMed 27748752
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Ppard aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P35396
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Reactivity data
Select an application| WB | ICC/IF | ELISA | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30000 - 1/70000 | Notes - |
Species Mouse | Dilution info 1/30000 - 1/70000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
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Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
Recommended products
Rabbit Polyclonal PPAR delta antibody. Suitable for WB, ICC/IF, ELISA, IHC-P and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ppard aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Ppard aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P35396
- Purification technique
- Affinity purification Immunogen
- Specificity
This affinity purified antibody is directed against mouse PPAR delta protein. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
- Concentration
- Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab8937)
ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-PPAR delta antibody (ab8937)
Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysateLane 1: ab8937 1/500
Lane 2: ab8937 1/1000Secondary antibody: Goat anti-Rabbit (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000
Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (NIH/3T3 whole cell lysate ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000
All lanes: Western blot - Anti-PPAR delta antibody (ab8937)
Predicted band size: 50 kDa
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