Rabbit Recombinant Monoclonal PPAR delta antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Images
![Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img20225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866)")
![Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img64922.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866)")
![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (AB178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--immunoprecipitation-img8473.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (AB178866)")
![Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img152154.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR delta antibody [EPR18517] (AB178866)")
Publications
Filter by
- Human molecular genetics 32:2219-22282023Breast cancer cell-derived exosome-delivered microRNA-155 targets UBQLN1 in adipocytes and facilitates cancer cachexia-related fat loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Sun,Z Wang,F Yao,K Sun,Z Li,S Sun,C LiPubMed 37017334
- Journal of orthopaedic surgery and research 18:212023Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuping Zhu,Wuping Li,Linhua Wang,Bing Dai,Zongyi Liu,Hang Wu,Ting DengPubMed 36624462
- Neurotoxicity research 40:2117-21312022Brucine-Induced Neurotoxicity by Targeting Caspase 3: Involvement of PPARγ/NF-κB/Apoptosis Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaying Lei,Fangqin Hou,Xiaoyu Wu,Yang Yi,Fan Xu,Qihai Gong,Jianmei GaoPubMed 36151391
- Cancer discovery 12:2414-24332022A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime Abrego,Hannah Sanford-Crane,Chet Oon,Xu Xiao,Courtney B Betts,Duanchen Sun,Shanthi Nagarajan,Luis Diaz,Holly Sandborg,Sohinee Bhattacharyya,Zheng Xia,Lisa M Coussens,Peter Tontonoz,Mara H ShermanPubMed 35894778
- Phytotherapy research : PTR 35:6932-6943202118β-glycyrrhetinic acid improves high-intensity exercise performance by promoting glucose-dependent energy production and inhibiting oxidative stress in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Ma,Hui Chen,Lixing Cao,Shuang Zhao,Chong Zhao,Shutao Yin,Hongbo HuPubMed 34709693
- Scientific reports 10:84282020PPARδ-mediated mitochondrial rewiring of osteoblasts determines bone mass.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorothea I H Müller et. al.PubMed 32439961
- eLife 6:2017Bladder-cancer-associated mutations in activate peroxisome proliferator-activated receptors to drive urothelial proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela M Halstead et. al.PubMed 29143738
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
Recommended products
Rabbit Recombinant Monoclonal PPAR delta antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18517
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img20225.jpg "Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)")
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)
Blocking/Dilution buffer: 5% NFDM/TBST.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR delta full length recombinant protein contains aa1-441 with a His-Tag.
All lanes: Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1: Human PPAR delta full length recombinant protein at 0.01 µg
Lane 2: Human PPAR alpha full length recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa
Exposure time: 5s
![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img64922.jpg "Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)")
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 15 seconds.
All lanes: Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 18 kDa, 24 kDa, 50 kDa, 80 kDa, 94 kDa
Observed band size: 30 kDa, 52 kDa, 80 kDa
![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--immunoprecipitation-img8473.jpg "Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)")
Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)
PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10μg (Input).
Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab178866 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)
Predicted band size: 50 kDa
![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-ab178866--western-blot-img152154.jpg "Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)")
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 10 seconds.
All lanes: Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat heart lysate at 10 µg
Lane 6: Rat kidney lysate at 10 µg
Lane 7: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 8: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa
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