Rabbit Recombinant Monoclonal PPAR delta antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Recombinant fragment, Rat, Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (AB223137), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-bsa-and-azide-free-ab223137--immunoprecipitation-img8473.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (AB223137)")
Publications
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- Molecular medicine reports 18:789-7982018Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR‑γ and UCP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wang et. al.PubMed 29845235
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Predicted | Expected |
| Mouse | Tested | Expected |
| Rat | Predicted | Expected |
| Recombinant fragment | Not recommended | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
Recommended products
Rabbit Recombinant Monoclonal PPAR delta antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Recombinant fragment, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18517
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab223137 is the carrier-free version of Anti-PPAR delta antibody [EPR18517] ab178866.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137), expandable thumbnail](https://content.abcam.com/products/images/ppar-delta-antibody-epr18517-bsa-and-azide-free-ab223137--immunoprecipitation-img8473.jpg "Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)")
Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with Anti-PPAR delta antibody [EPR18517] ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using Anti-PPAR delta antibody [EPR18517] ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10μg (Input).
Lane 2: Anti-PPAR delta antibody [EPR18517] ab178866 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PPAR delta antibody [EPR18517] ab178866 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPAR delta antibody [EPR18517] ab178866).
All lanes: Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (Anti-PPAR delta antibody [EPR18517] ab178866)
Predicted band size: 50 kDa
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