Mouse Monoclonal PPAR gamma antibody. Suitable for IP, WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples. Cited in 53 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPARG aa 1-150.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118402.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928)")
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunoprecipitation-img1743.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928)")
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--western-blot-img73072.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125440.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (AB41928)")
Publications
Filter by
- Frontiers in endocrinology 14:12122912023PM induced liver lipid metabolic disorders in C57BL/6J mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxiao Zhang,Tengfei Ma,Chang Liu,Ding Ma,Jian Wang,Meng Liu,Jinjun Ran,Xueting Wang,Xiaobei DengPubMed 37780625
- Stem cell research & therapy 13:3422022C/EBPα regulates the fate of bone marrow mesenchymal stem cells and steroid-induced avascular necrosis of the femoral head by targeting the PPARγ signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Duan,Hanyu Wang,Xinzeyu Yi,Hao Zhang,Hui Chen,Zhenyu PanPubMed 35883192
- Bone & joint research 11:453-4642022GDF11 inhibits abnormal adipogenesis of condylar chondrocytes in temporomandibular joint osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelin Wang,Yuqian Shi,Feng He,Tao Ye,Shibin Yu,Hui Miao,Qian Liu,Mian ZhangPubMed 35787089
- Cell death discovery 8:1602022Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi HuPubMed 35379779
- iScience 25:1041842022Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeroy C Joseph,Jianting Shi,Quynh N Nguyen,Victoria Pensiero,Chris Goulbourne,Robert C Bauer,Hanrui Zhang,John P MorrowPubMed 35494220
- The heart surgery forum 25:E124-E1312022Atorvastatin Exerts Protective Effect on Cardiopulmonary Bypass Induced Renal Injury in Rats via PPAR-γ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Jianjun Ge,Can WeiPubMed 35238313
- Experimental and therapeutic medicine 23:2732022Role of HSP90α in osteoclast formation and osteoporosis development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianli Ma,Chen Yang,Huajian Zhong,Cheng Wang,Ke Zhang,Xiaoming Li,Jinhui Wu,Yang GaoPubMed 35251339
- Molecular therapy. Nucleic acids 26:1303-13172021Ubiquitinated ligation protein NEDD4L participates in MiR-30a-5p attenuated atherosclerosis by regulating macrophage polarization and lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Song,Jing-Zhou Li,Yao Wu,Wei-Yin Wu,Yan Wang,Gang LiPubMed 34853729
- Journal of bioenergetics and biomembranes 53:573-5832021Rosuvastatin alleviated the liver ischemia reperfusion injury by activating the expression of peroxisome proliferator-activated receptor gamma (PPARγ).Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuiyuan Guo,Jie CaiPubMed 34235609
- International journal of molecular sciences 22:2021Chronic Low-Dose Alcohol Consumption Attenuates Post-Ischemic Inflammation via PPARγ in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Li,Jiyu Li,Ethyn G Loreno,Sumitra Miriyala,Manikandan Panchatcharam,Xiaohong Lu,Hong SunPubMed 34066125
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PPARG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P37231
Abcam Recommends
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes A nuclear extraction is recommended. |
Species Human | Dilution info - | Notes A nuclear extraction is recommended. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform antigen retrieval by autoclave to preserve reproducibility. |
Species Human | Dilution info - | Notes Perform antigen retrieval by autoclave to preserve reproducibility. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity). (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Additional Targets
PPARG
Alternative names
NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3
Recommended products
Mouse Monoclonal PPAR gamma antibody. Suitable for IP, WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples. Cited in 53 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPARG aa 1-150.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PPARG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P37231
- Clone number
- A3409A
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118402.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)
ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunoprecipitation-img1743.jpg "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)")
Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)
Lane 1: FLAG-M2
Lane 2: ab41928
Lane 3: ab41928
Lane 4: Control IgGImmunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4°C for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.
This image was generated using the ascites version of the product.
All lanes: Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--western-blot-img73072.jpg "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)")
Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)
Two bands are visible: PPAR gamma 1 and PPAR gamma 2.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928) at 1 µg/mL
Lane 1: Nuclear lysate of non induced 3T3l-1 cells at 20 µg
Lane 2: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation at 20 µg
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-a3409a-ab41928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125440.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [A3409A] (ab41928)
ab41928 staining PPAR gamma 1+2 in rat plancenta (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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