Rabbit Recombinant Monoclonal PPAR gamma antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunoprecipitation-img416488.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324)")
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunoprecipitation-img416487.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324)")
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--western-blot-img416452.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324)")
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--western-blot-img416451.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416558.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (AB310324)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Expected | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity). (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Alternative names
NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3
Recommended products
Rabbit Recombinant Monoclonal PPAR gamma antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25862-79
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunoprecipitation-img416488.jpg "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
PPAR gamma 1+2 was immunoprecipitated from 0.35 mg 3T3-L1 differentiation for 6 days whole cell lysate with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 3T3-L1 differentiation for 6 days whole cell lysate
Lane 2: Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 IP in 3T3-L1 differentiation for 6 days whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 in 3T3-L1 differentiation for 6 days whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 87 seconds
The bands beneath the target band (53kDa) are expected to be degraded target fragments.All lanes: Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] (Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323) at 1/30 dilution
All lanes: 3T3-L1 differentiation for 6 days whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 87s
![Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunoprecipitation-img416487.jpg "Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
PPAR gamma 1+2 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 2: Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 IP in HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 in HDLM-2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 secondsAll lanes: Immunoprecipitation - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] (Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323) at 1/30 dilution
All lanes: HDLM-2 (human Hodgkin lymphoma) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--western-blot-img416452.jpg "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human PPARD and PPARA.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] (Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323) at 1/1000 dilution
Lane 1: His-tagged human PPARA recombinant protein at 10 ng
Lane 2: His-tagged human PPARD recombinant protein at 10 ng
Lane 3: His-tagged human PPARG recombinant fragment at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 37 kDa
Exposure time: 15s
![Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--western-blot-img416451.jpg "Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: MOLT-4 (PMID: 7787419).
The expression of PPARG is upregulated in response to 3T3-L1 differentiation for 6 days treatment (PMID: 9065481).
The bands beneath the target band (53kDa) are expected to be degraded target fragments.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 37 secondsAll lanes: Western blot - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] (Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323) at 1/1000 dilution
Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 50 µg
Lane 2: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 50 µg
Lane 3: 3T3-L1 differentiation for 6 days whole cell lysate at 50 µg
Lane 4: MOLT-4 whole cell lysate at 50 µg
Lane 5: Mouse brown fat tissue lysate at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 53 kDa, 58 kDa
Exposure time: 37s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416558.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human cerebrum. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416557.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Panel A 3T3-L1(mou tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on (A) 3T3-L1 cell pellet differentiated for 6 days; no staining on (B) undifferentiated 3T3-L1 cell pellet. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416556.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat brown adipose tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat brown adipose. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416555.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat white adipose tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat white adipose. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416554.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse brown adipose tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse brown adipose. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse white adipose tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse white adipose. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416552.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)
This data was developed using Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human white adipose tissue labeling PPAR gamma 1+2 with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 at 1/200 (2.575 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human white adipose. The section was incubated with Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] ab310323 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416557.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416556.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416555.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416554.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324), expandable thumbnail](https://content.abcam.com/products/images/ppar-gamma-2-ppar-gamma-antibody-epr25862-79-bsa-and-azide-free-ab310324--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416552.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma 2 + PPAR gamma antibody [EPR25862-79] - BSA and Azide free (ab310324)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com