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AB324248

Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal PPAR gamma antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3

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Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)

Interpolated concentration of native PPAR gamma was measured at different sample concentrations. Undiluted samples are as follows : LoVo cell extract (62.5 µg/mL) and Capan-1 cell extract (125 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)

Sandwich ELISA with the capture antibody ab324248 dilution at 2 ug/mL and detector antibody ab324249 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)

Interpolated concentration of native PPAR gamma was measured at different sample concentrations. Undiluted sample is as follows : rat brown adipose tissue extract (200 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PPAR gamma antibody [EPR29314-24] - BSA and Azide free (Capture) (AB324248)

Interpolated concentration of native PPAR gamma was measured at different sample concentrations. Undiluted samples are as follows : 3T3-L1 cell extract (differentiated for 6 days) (50 µg/mL) and mouse brown adipose tissue extract (100 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29314-24

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 17% cross reaction with human PPAR alpha, but does not cross-react with human PPAR delta.

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Reactivity data

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Product details

ab324248 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human, Mouse, Rat PPAR gamma. The recommended pair for sandwich ELISA is:

Capture: ab324248, Human, Mouse, Rat PPAR gamma Capture Antibody (unconjugated)

Detector: ab324249, Human, Mouse, Rat PPAR gamma Detector Antibody (unconjugated)

The reference range value is 78.1-5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity).. (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
See full target information PPARG
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Product promise

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