Anti-PPAR gamma antibody

• WB

AbReview35975****

Western blot - Anti-PPAR gamma antibody (AB45036)

All lanes:

Western blot - Anti-PPAR gamma antibody (ab45036)

Lane 1:

Protein Marker

Lane 2:

Mouse liver tissue lysate at 1 µg

Secondary

All lanes:

IRDye® 680RD Donkey anti-Rabbit

Predicted band size: 57 kDa

false

Exposure time: 2s

This image is courtesy of Xiaofeng Xin

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB45036)

ab45036 positive staining PPAR gamma in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB45036)

ab45036 staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1 : 200) for 1 hour at room temperature. A Dylight 680-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/1000).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB45036)

ab45036 positive staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB45036)

Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB45036)

ab45036 positive staining PPAR gamma in NIH-3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.

• WB

Supplier Data

Western blot - Anti-PPAR gamma antibody (AB45036)

PPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.

All lanes:

Western blot - Anti-PPAR gamma antibody (ab45036) at 1/500 dilution

Lane 1:

3T3 at 20 µg

Lane 2:

3T3-L1 differentiated day 7 at 20 µg

Predicted band size: 57 kDa

false