Anti-PPAR gamma antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma antibody (AB59256)

ab59256, at 1/50 dilution, staining PPAR gamma in paraffin embedded human placenta tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (AB59256)

ICC/IF image of ab59256 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

Supplier Data

Western blot - Anti-PPAR gamma antibody (AB59256)

All lanes:

Western blot - Anti-PPAR gamma antibody (ab59256) at 1/500 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell line) whole cell lysate

Lane 2:

HUVEC (blocked with immunizing peptide), whole cell lysate

Predicted band size: 57 kDa

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