AB178860

Anti-PPAR gamma antibody [EPR18516]

RabMAb
Recombinant
What is this?

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(81 Publications)

Anti-PPAR gamma antibody [EPR18516] (ab178860) is a rabbit monoclonal antibody detecting PPAR gamma in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

View Alternative Names

NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (AB178860)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PPAR gamma with ab178860 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weak cytoplasmic staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling PPAR gamma with ab178860 at 1/1500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PPAR gamma with ab178860 at 1/1500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling PPAR gamma with ab178860 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and weak cytoplasmic staining on K562 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Western blot - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 seconds; Lane 2 : 3 seconds.

All lanes:

Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860) at 1/1000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

Supplier Data

Western blot - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPAR gamma antibody [EPR18516] (ab178860) at 1/1000 dilution

Lane 1:

Human adipose tissue lysate at 20 µg

Lane 2:

Human ovary lysate at 20 µg

Lane 3:

Human placenta lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

Exposure time: 3min

Carrier free

Anti-PPAR gamma antibody [EPR18516] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PPAR gamma antibody [EPR18516]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PPAR gamma antibody [EPR18516]
660 APC

APC Anti-PPAR gamma antibody [EPR18516]
578 PE

PE Anti-PPAR gamma antibody [EPR18516]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PPAR gamma antibody [EPR18516]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PPAR gamma antibody [EPR18516]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18516

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Expression levels of PPARy protein vary with sample type. Induction may be required if endogenous expression is low.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1500", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PPAR gamma antibody [EPR18516] (ab178860) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of PPAR gamma?
Anti-PPAR gamma [EPR18516] (ab178860) specifically detects a band for PPAR gamma (UniProt: P37231) at a molecular weight of 58kDa.

Trusted by the scientific community
Anti-PPAR gamma [EPR18516] (ab178860) was first used in a scientific publication in 2017 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR18516] also available for your convenience: ab178860, Carrier free - ab238441, APC - ab310835, PE - ab310910, Alexa Fluor® 647 - ab311099, Alexa Fluor® 594 - ab311714, Alexa Fluor® 568 - ab312990, Alexa Fluor® 555 - ab313195

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity).. (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.

See full target information PPARG

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Publications (81)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 24:307 PubMed41039585

2025

MLN4924 inhibits macrophage foam cell formation by enhancing autophagy and regulating metabolic phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xing,Yu Bu,Jingqi Shi,Jing Liu,Fan Hu,Minghua Zhang,Jiao Fan

Balkan medical journal 42:538-546 PubMed41035257

2025

Fat Mass and Obesity-Associated Protein-Mediated Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Promotes Chemotherapy Resistance in Acute Myeloid Leukemia

Applications

Unspecified application

Species

Unspecified reactive species

Pan Zhipeng,Kang Lixia,Chen Ling

Orphanet journal of rare diseases 20:453 PubMed40849475

2025

Alpha-lipoic acid supplementation improves pathological alterations in cellular models of Friedreich ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Talaverón-Rey,Diana Reche-López,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,David Gómez-Fernández,Ana Romero-González,Paula Cilleros-Holgado,José Manuel Romero-Domínguez,Alejandra López-Cabrera,Rocío Piñero-Pérez,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

Discover oncology 16:1103 PubMed40515805

2025

LMNB1 regulates breast cancer cell senescence and migration through PPAR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoda Meng,Sicheng Liu,Chunsheng Liu,Kunxian Yang,Minghua Lai

Molecular medicine (Cambridge, Mass.) 31:206 PubMed40414869

2025

METTL9 mediated N1-Histidine methylation of SLC39A7 confers ferroptosis resistance and inhibits adipogenic differentiation in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahao Jin,Quanfeng Li,Yunhui Zhang,Pengfei Ji,Xinlang Wang,Yibin Zhang,Zihao Yuan,Jianan Jiang,Guangqi Tian,Mingxi Cai,Pei Feng,Yanfeng Wu,Peng Wang,Wenjie Liu

Cellular and molecular life sciences : CMLS 82:149 PubMed40192802

2025

Circular RNA circSTX12 regulates osteo-adipogenic balance and proliferation of BMSCs in senile osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Gu,Wenhui Yu,Pei Feng,Chenying Zeng,Qian Cao,Fenglei Chen,Ziming Wang,Huiyong Shen,Yanfeng Wu,Shan Wang

Journal of orthopaedic surgery and research 20:260 PubMed40069718

2025

Therapeutic potential of total flavonoids of Rhizoma Drynariae: inhibiting adipogenesis and promoting osteogenesis via MAPK/HIF-1α pathway in primary osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Su,Luyao Liu,Zechen Yan,WenXuan Guo,Guangxin Huang,Rujie Zhuang,Yu Pan

Animals : an open access journal from MDPI 15: PubMed40002988

2025

Whole Transcriptome Sequencing Reveals miRNAs and ceRNA Networks in Duck Abdominal Fat Deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiu Wang,Chunyan Yang,Bingqiang Dong,Anqi Chen,Qianqian Song,Hao Bai,Yong Jiang,Guobin Chang,Guohong Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2417326 PubMed39996504

2025

Lipid Droplets Metabolism Mediated by ANXA7-PPARγ Signaling Axis Regulates Spinal Cord Injury Repair in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Haoran Liu,Linlin Jiang,Zihang Wang,Yong Chang,Na Li,Shiqing Feng

Nature communications 16:1499 PubMed39929846

2025

Transdermal microneedle-assisted ultrasound-enhanced CRISPRa system to enable sono-gene therapy for obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyue Li,Jifeng Yu,Yuting Shen,Bing Xiong,De Zhao,Weichen Xu,Shen Zhang,Xin Guan,Yunyun Liu,Xuexia Shan,Anqi Zhu,Qi Lyu,Yan Fang,Zitong Chen,Haohao Yin,Liping Sun,Huixiong Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPAR gamma antibody [EPR18516]

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Western blot - Anti-PPAR gamma antibody [EPR18516] (AB178860)

Western blot - Anti-PPAR gamma antibody [EPR18516] (AB178860)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (81)

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