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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Multiclonal
WB | IP | Flow Cyt (Intra) | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Expected | Not recommended | Not recommended |
Rat | Tested | Expected | Expected | Not recommended | Not recommended |
Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).(Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG
Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Multiclonal
RM1091
Affinity purification Protein A
Unsuitable for mouse IP.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution
Lane 1: His-tagged PPAR delta recombinant protein at 10 ng
Lane 2: His-tagged PPAR alpha recombinant protein at 10 ng
Lane 3: His-GST-tagged PPAR gamma recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 85 kDa
Exposure time: 180s
Anti-PPAR gamma antibody (ab316981) staining at 1/1000 dilution is specific to human, mouse and rat species. Anti-PPAR gamma antibody (ab209350) at 1/1000 dilution is bandy than ab316981.
The expression profile observed is consistent with what has been described in the literature (PMID: 9065481).
The bands beneath the target band (53kDa) are expected to be degraded target fragments.
All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution
Lane 1: Human fat tissue lysate at 20 µg
Lane 2: Mouse brown fat tissue lysate at 20 µg
Lane 3: Mouse ovary tissue lysate at 20 µg
Lane 4: Mouse colon cancer tissue lysate at 20 µg
Lane 5: Mouse skeletal muscle tissue lysate at 20 µg
Lane 6: Rat brown fat tissue lysate at 20 µg
Lane 7: Rat ovary tissue lysate at 20 µg
Lane 8: Rat colon tissue lysate at 20 µg
Lane 9: Rat skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 53 kDa, 58 kDa
Exposure time: 180s
Anti-PPAR gamma antibody (ab316981) staining at 1/1000 dilution is specific to human, mouse and rat species. Anti-PPAR gamma antibody (ab209350) at 1/1000 dilution is bandy than ab316981.
The expression profile observed is consistent with what has been described in the literature (PMID: 9065481).
The bands beneath the target band (53kDa) are expected to be degraded target fragments.
Negative control: NIH/3T3 (PMID:12023027).
The expression of PPARG is upregulated in response to 3T3-L1 differentiation for 6 days treatment (PMID: 28759152).
The bands beneath the target band (53kDa) are expected to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution
Lane 1: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: 3T3-L1 differentiation for 6 days whole cell lysate at 20 µg
Lane 3: NIH/3T3 at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 53 kDa, 58 kDa, 36 kDa
Exposure time: 180s
Negative control: MOLT-4 and Jurkat (PMID: 7787419).
The expression of PPARG is upregulated in response to TPA treatment ( PMID: 9568716/PMID: 19850645).
The bands beneath the target band (53kDa) are expected to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution
Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg
Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 3 - 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 5: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 6: THP-1 treated with 80nM TPA for 24 hours, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Observed band size: 53 kDa, 58 kDa, 36 kDa
Exposure time: 180s
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) (Right) / Jurkat(human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling PPAR gamma with ab316981 at 1/50 dilution (1ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: Jurkat (PMID: 7787419).
Anti-PPAR gamma antibody (ab316981) is specific to human species, while Anti-PPAR gamma antibody (ab209350) is non-specific.
PPAR gamma was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab316981 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316981 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab316981 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab316981 in PC-3 whole cell lysate
All lanes: Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/30 dilution
All lanes: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution
Exposure time: 180s
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