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Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981), expandable thumbnail
  • Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981), expandable thumbnail
  • Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981), expandable thumbnail
  • Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [RM1091] (AB316981), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity). (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.

Alternative names

NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3

Recommended products

Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1091
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse IP.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981) at 1/1000 dilution

    Lane 1: His-tagged PPAR delta recombinant protein at 10 ng with 5% NFDM/TBST

    Lane 2: His-tagged PPAR alpha recombinant protein at 10 ng with 5% NFDM/TBST

    Lane 3: His-GST-tagged PPAR gamma recombinant protein at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 85 kDa

    Exposure time: 180s

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Anti-PPAR gamma antibody (ab316981) staining at 1/1000 dilution is specific to human, mouse and rat species. Anti-PPAR gamma antibody (Anti-PPAR gamma antibody ab209350) at 1/1000 dilution is bandy than ab316981.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9065481).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981) at 1/1000 dilution

    Lane 1: Human fat tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse brown fat tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Mouse ovary tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse colon cancer tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Rat brown fat tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 7: Rat ovary tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 8: Rat colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 9: Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa

    Exposure time: 180s

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Negative control: NIH/3T3 (PMID:12023027).

    The expression of PPARG is upregulated in response to 3T3-L1 differentiation for 6 days treatment (PMID: 28759152).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981) at 1/1000 dilution

    Lane 1: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 3T3-L1 differentiation for 6 days whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: NIH/3T3 at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Negative control: MOLT-4 and Jurkat (PMID: 7787419).

    The expression of PPARG is upregulated in response to TPA treatment ( PMID: 9568716/PMID: 19850645).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lanes 3 - 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 6: THP-1 treated with 80nM TPA for 24 hours, whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa, 36 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) (Right) / Jurkat(human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling PPAR gamma with ab316981 at 1/50 dilution (1ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID: 7787419).

    Anti-PPAR gamma antibody (ab316981) is specific to human species, while Anti-PPAR gamma antibody (Anti-PPAR gamma antibody ab209350) is non-specific.

  • Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (ab316981)

    PPAR gamma was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab316981 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316981 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab316981 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316981 in PC-3 whole cell lysate

    All lanes: Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (ab316981) at 1/30 dilution

    All lanes: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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