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Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).(Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.

Alternative names

Recommended products

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Rabbit Recombinant Multiclonal PPAR gamma antibody. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1091

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for mouse IP.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution

    Lane 1: His-tagged PPAR delta recombinant protein at 10 ng

    Lane 2: His-tagged PPAR alpha recombinant protein at 10 ng

    Lane 3: His-GST-tagged PPAR gamma recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 85 kDa

    Exposure time: 180s

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Anti-PPAR gamma antibody (ab316981) staining at 1/1000 dilution is specific to human, mouse and rat species. Anti-PPAR gamma antibody (ab209350) at 1/1000 dilution is bandy than ab316981.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9065481).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution

    Lane 1: Human fat tissue lysate at 20 µg

    Lane 2: Mouse brown fat tissue lysate at 20 µg

    Lane 3: Mouse ovary tissue lysate at 20 µg

    Lane 4: Mouse colon cancer tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 6: Rat brown fat tissue lysate at 20 µg

    Lane 7: Rat ovary tissue lysate at 20 µg

    Lane 8: Rat colon tissue lysate at 20 µg

    Lane 9: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa

    Exposure time: 180s

    Anti-PPAR gamma antibody (ab316981) staining at 1/1000 dilution is specific to human, mouse and rat species. Anti-PPAR gamma antibody (ab209350) at 1/1000 dilution is bandy than ab316981.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9065481).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Negative control: NIH/3T3 (PMID:12023027).

    The expression of PPARG is upregulated in response to 3T3-L1 differentiation for 6 days treatment (PMID: 28759152).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution

    Lane 1: Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: 3T3-L1 differentiation for 6 days whole cell lysate at 20 µg

    Lane 3: NIH/3T3 at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Western blot - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Negative control: MOLT-4 and Jurkat (PMID: 7787419).

    The expression of PPARG is upregulated in response to TPA treatment ( PMID: 9568716/PMID: 19850645).

    The bands beneath the target band (53kDa) are expected to be degraded target fragments.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3 - 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 5: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 6: THP-1 treated with 80nM TPA for 24 hours, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 53 kDa, 58 kDa, 36 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PPAR gamma antibody [RM1091] (ab316981)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) (Right) / Jurkat(human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling PPAR gamma with ab316981 at 1/50 dilution (1ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID: 7787419).

    Anti-PPAR gamma antibody (ab316981) is specific to human species, while Anti-PPAR gamma antibody (ab209350) is non-specific.

  • Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (ab316981), expandable thumbnail

    Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (ab316981)

    PPAR gamma was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab316981 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316981 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab316981 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab316981 in PC-3 whole cell lysate

    All lanes: Immunoprecipitation - Anti-PPAR gamma antibody [RM1091] (AB316981) at 1/30 dilution

    All lanes: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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