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AB202064

Anti-PPCEL/PREPL antibody [EPR17751]

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(2 Publications)

Rabbit Recombinant Monoclonal PPCEL/PREPL antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

KIAA0436, PREPL, Prolyl endopeptidase-like, Prolylendopeptidase-like

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab202064 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab202064 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab202064 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 (red).
The negative controls are as follows : -
-ve control 1 - ab202064 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
-ve control 2. - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • IP

Supplier Data

Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

PPCEL/PREPL was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell extract with ab202064 at 1/40.

Western blot was performed using ab202064 at 1/2000.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : MCF7 whole cell lysate at 10ug.

Lane 2 : Immunoprecipitate of MCF7 whole cell lysate using ab202064.

Lane 3 : Immunoprecipitate of MCF7 whole cell lysate using ab172730 (Rabbit monoclonal IgG).
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)

Predicted band size: 84 kDa

Observed band size: 72 kDa

true

Exposure time: 8s

Flow Cytometry (Intracellular) - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast) cells labeling PPCEL/PREPL with ab202064 at 1/150 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • WB

Supplier Data

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Blocking and diluting buffer was 5% NFDM/TBST

All lanes:

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution

Lane 1:

A673 (Human muscle Ewing's Sarcoma cell line) lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line ) lysate at 20 µg

Lane 3:

L6 (Rat skeletal muscle cell line) lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 72 kDa

true

Exposure time: 15s

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • WB

Supplier Data

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Blocking and diluting buffer was 5% NFDM/TBS

All lanes:

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 72 kDa

true

Exposure time: 15s

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • WB

Supplier Data

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Blocking and diluting buffer was 5% NFDM/TBS

All lanes:

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) lysate at 10 µg

Lane 2:

RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 72 kDa

true

Exposure time: 5s

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • WB

Supplier Data

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Blocking and diluting buffer was 5% NFDM/TBS

All lanes:

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution

All lanes:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 72 kDa

true

Exposure time: 5s

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)
  • WB

Supplier Data

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

Blocking and diluting buffer was 5% NFDM/TBS

All lanes:

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/10000 dilution

All lanes:

Recombinant Human PPCEL/PREPL protein (<a href='/en-us/products/unavailable/recombinant-human-ppcelprepl-protein-ab160557'>ab160557</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 84 kDa

Observed band size: 110 kDa

true

Exposure time: 3s

  • Carrier free

    Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17751

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine peptidase whose precise substrate specificity remains unclear (PubMed : 16143824, PubMed : 16385448, PubMed : 28726805). Does not cleave peptides after a arginine or lysine residue (PubMed : 16143824). Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex (PubMed : 23321636). May play a role in the regulation of synaptic vesicle exocytosis (PubMed : 24610330).
See full target information PREPL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37833926

2023

RAD51D Secondary Mutation-Mediated Resistance to PARP-Inhibitor-Based Therapy in HGSOC.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Yilin Dai,Yi Gao,Ranran Chai,Chong Lu,Bing Yu,Yu Kang,Congjian Xu

Nature cell biology 25:1575-1589 PubMed37770567

2023

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Kramer,Gyan Prakash,R Stefan Isaac,Karine Choquet,Iliana Soto,Boryana Petrova,Hope E Merens,Naama Kanarek,L Stirling Churchman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com