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AB140626

Anti-PPCS antibody [EPR9453]

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(2 Publications)

Rabbit Recombinant Monoclonal PPCS antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

COAB, PPCS, Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase

1 Images
Western blot - Anti-PPCS antibody [EPR9453] (AB140626)
  • WB

Unknown

Western blot - Anti-PPCS antibody [EPR9453] (AB140626)

All lanes:

Western blot - Anti-PPCS antibody [EPR9453] (ab140626) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 34 kDa

false

  • Carrier free

    Anti-PPCS antibody [EPR9453] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9453

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine (PubMed : 11923312, PubMed : 12906824, PubMed : 29754768). Has a preference for ATP over CTP as a cosubstrate (PubMed : 11923312).
See full target information PPCS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications medicine 5:323 PubMed40745475

2025

Pantethine ameliorates dilated cardiomyopathy features in PPCS deficiency disorder in patients and cell line models.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Zhang,Tatjana Dorn,Barbara Gnutti,Yair Anikster,Sarah Kuebler,Rebecca Ahrens-Nicklas,Rachel Gosselin,Shamima Rahman,Ronen Durst,Enrica Zanuttigh,Miriam A Güra,Christine M Poch,Anna B Meier,Karl-Ludwig Laugwitz,Hans-Joachim Schüller,Ana C Messias,Ody C Sibon,Dario Finazzi,Alyssa Rippert,Dong Li,Kristen Truxal,Deipanjan Nandi,Brent C Lampert,Mildrid Yeo,Alice Gardham,Batel Nissan,Smadar Horowitz Cederboim,Alessandra Moretti,Arcangela Iuso

American journal of human genetics 102:1018-1030 PubMed29754768

2018

Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Arcangela Iuso,Marit Wiersma,Hans-Joachim Schüller,Ben Pode-Shakked,Dina Marek-Yagel,Mathias Grigat,Thomas Schwarzmayr,Riccardo Berutti,Bader Alhaddad,Bart Kanon,Nicola A Grzeschik,Jürgen G Okun,Zeev Perles,Yishay Salem,Ortal Barel,Amir Vardi,Marina Rubinshtein,Tal Tirosh,Gal Dubnov-Raz,Ana C Messias,Caterina Terrile,Iris Barshack,Alex Volkov,Camilla Avivi,Eran Eyal,Elisa Mastantuono,Muhamad Kumbar,Shachar Abudi,Matthias Braunisch,Tim M Strom,Thomas Meitinger,Georg F Hoffmann,Holger Prokisch,Tobias B Haack,Bianca J J M Brundel,Dorothea Haas,Ody C M Sibon,Yair Anikster
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com