Rabbit Recombinant Monoclonal PPCS antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (AB175048), expandable thumbnail](https://content.abcam.com/products/images/ppcs-antibody-epr9453-bsa-and-azide-free-ab175048--western-blot-img62051.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (AB175048)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine (PubMed:11923312, PubMed:12906824, PubMed:29754768). Has a preference for ATP over CTP as a cosubstrate (PubMed:11923312).
Alternative names
COAB, PPCS, Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase
Recommended products
Rabbit Recombinant Monoclonal PPCS antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9453
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab175048 is the carrier-free version of Anti-PPCS antibody [EPR9453] ab140626.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (ab175048), expandable thumbnail](https://content.abcam.com/products/images/ppcs-antibody-epr9453-bsa-and-azide-free-ab175048--western-blot-img62051.jpg "Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (ab175048)")
Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (ab175048)
This data was developed using Anti-PPCS antibody [EPR9453] ab140626, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PPCS antibody [EPR9453] (Anti-PPCS antibody [EPR9453] ab140626) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: SH-SY5Y cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Predicted band size: 34 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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