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AB175048

Anti-PPCS antibody [EPR9453] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPCS antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

COAB, PPCS, Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase

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Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (AB175048)
  • WB

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Western blot - Anti-PPCS antibody [EPR9453] - BSA and Azide free (AB175048)

This data was developed using ab140626, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPCS antibody [EPR9453] (<a href='/en-us/products/primary-antibodies/ppcs-antibody-epr9453-ab140626'>ab140626</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9453

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab175048 is the carrier-free version of ab140626.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine (PubMed : 11923312, PubMed : 12906824, PubMed : 29754768). Has a preference for ATP over CTP as a cosubstrate (PubMed : 11923312).
See full target information PPCS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com