AB69569

Anti-PPHLN1/Periphilin-1 antibody

Name: Anti-PPHLN1/Periphilin-1 antibody (ab69569) | Abcam
Brand: Abcam Limited
SKU: AB69569
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal PPHLN1/Periphilin-1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

HSPC206, HSPC232, PPHLN1, Periphilin-1, CDC7 expression repressor, Gastric cancer antigen Ga50, CR

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPHLN1/Periphilin-1 antibody (AB69569)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPHLN1/Periphilin-1 antibody (AB69569)

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.

Unknown

Western blot - Anti-PPHLN1/Periphilin-1 antibody (AB69569)

All lanes:

Western blot - Anti-PPHLN1/Periphilin-1 antibody (ab69569) at 1/500 dilution

Lane 1:

Extracts from COLO cells at 30 µg

Lane 2:

Extracts from 293 cells at 30 µg

Lane 3:

Extracts from COLO cells at 30 µg with immunizing peptide

Predicted band size: 53 kDa

Observed band size: 100 kDa,35 kDa,40 kDa,53 kDa,55 kDa,85 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab69569 detects endogenous levels of total PPHLN protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding component of the HUSH complex, a multiprotein complex that mediates epigenetic repression of mobile genetic elements, such as retroviruses and transposable elements (PubMed : 15474462, PubMed : 17963697, PubMed : 26022416, PubMed : 32976585, PubMed : 39658355). The HUSH complex mainly represses LINE-1 (L1) retrotransposons that are still capable of transposition (PubMed : 32976585, PubMed : 39658355). The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2, a chromatin remodeler that compacts chromatin (PubMed : 26022416). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA : some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed : 30487602). Within the HUSH complex, PPHLN1 acts as a mRNA-binding component, which specifically binds nascent transcripts of mobile genetic elements, enabling HUSH-dependent silencing of transcripts (PubMed : 39658355). Contributes to the maintenance of the HUSH complex at chromatin (PubMed : 26022416, PubMed : 39013473). As part of the HUSH2 complex, promotes epigenetic repression of interferon-stimulated genes (PubMed : 33144593, PubMed : 39013473). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (PubMed : 12853457).

See full target information PPHLN1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bone & joint research 11:260-269 PubMed35502760

2022

Intermittent parathyroid hormone increases stability and improves osseointegration of initially unstable implants.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Staats,Branden R Sosa,Emile-Victor Kuyl,Yingzhen Niu,Vincentius Suhardi,Kathleen Turajane,Reinhard Windhager,Matthew B Greenblatt,Lionel Ivashkiv,Mathias P G Bostrom,Xu Yang

Journal of virology 96:e0203921 PubMed34878926

2021

Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Anshuman Das,Madhuvanthi Vijayan,Eric M Walton,V Grace Stafford,David N Fiflis,Aravind Asokan

Nature communications 11:4940 PubMed33009411

2020

TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher H Douse,Iva A Tchasovnikarova,Richard T Timms,Anna V Protasio,Marta Seczynska,Daniil M Prigozhin,Anna Albecka,Jane Wagstaff,James C Williamson,Stefan M V Freund,Paul J Lehner,Yorgo Modis

Scientific reports 7:10213 PubMed28860464

2017

Characterization of a core region in the A2UCOE that confers effective anti-silencing activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Zhang,Giorgia Santilli,Adrian J Thrasher

Breast cancer research : BCR 13:R40 PubMed21466675

2011

Exploring the link between MORF4L1 and risk of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Griselda Martrat,Christopher M Maxwell,Emiko Tominaga,Montserrat Porta-de-la-Riva,Núria Bonifaci,Laia Gómez-Baldó,Massimo Bogliolo,Conxi Lázaro,Ignacio Blanco,Joan Brunet,Helena Aguilar,Juana Fernández-Rodríguez,Sheila Seal,Anthony Renwick,Nazneen Rahman,Julia Kühl,Kornelia Neveling,Detlev Schindler,María J Ramírez,María Castellà,Gonzalo Hernández,Douglas F Easton,Susan Peock,Margaret Cook,Clare T Oliver,Debra Frost,Radka Platte,D Gareth Evans,Fiona Lalloo,Rosalind Eeles,Louise Izatt,Carol Chu,Rosemarie Davidson,Kai-Ren Ong,Jackie Cook,Fiona Douglas,Shirley Hodgson,Carole Brewer,Patrick J Morrison,Mary Porteous,Paolo Peterlongo,Siranoush Manoukian,Bernard Peissel,Daniela Zaffaroni,Gaia Roversi,Monica Barile,Alessandra Viel,Barbara Pasini,Laura Ottini,Anna Laura Putignano,Antonella Savarese,Loris Bernard,Paolo Radice,Sue Healey,Amanda Spurdle,Xiaoqing Chen,Jonathan Beesley,Matti A Rookus,Senno Verhoef,Madeleine A Tilanus-Linthorst,Maaike P Vreeswijk,Christi J Asperen,Danielle Bodmer,Margreet G E M Ausems,Theo A van Os,Marinus J Blok,Hanne E J Meijers-Heijboer,Frans B L Hogervorst,David E Goldgar,Saundra Buys,Esther M John,Alexander Miron,Melissa Southey,Mary B Daly,Katja Harbst,Ake Borg,Johanna Rantala,Gisela Barbany-Bustinza,Hans Ehrencrona,Marie Stenmark-Askmalm,Bella Kaufman,Yael Laitman,Roni Milgrom,Eitan Friedman,Susan M Domchek,Katherine L Nathanson,Timothy R Rebbeck,Oskar Thor Johannsson,Fergus J Couch,Xianshu Wang,Zachary Fredericksen,Daniel Cuadras,Víctor Moreno,Friederike K Pientka,Reinhard Depping,Trinidad Caldés,Ana Osorio,Javier Benítez,Juan Bueren,Tuomas Heikkinen,Heli Nevanlinna,Ute Hamann,Diana Torres,Maria Adelaide Caligo,Andrew K Godwin,Evgeny N Imyanitov,Ramunas Janavicius,Olga M Sinilnikova,Dominique Stoppa-Lyonnet,Sylvie Mazoyer,Carole Verny-Pierre,Laurent Castera,Antoine de Pauw,Yves-Jean Bignon,Nancy Uhrhammer,Jean-Philippe Peyrat,Philippe Vennin,Sandra Fert Ferrer,Marie-Agnès Collonge-Rame,Isabelle Mortemousque,Lesley McGuffog,Georgia Chenevix-Trench,Olivia M Pereira-Smith,Antonis C Antoniou,Julián Cerón,Kaoru Tominaga,Jordi Surrallés,Miguel Angel Pujana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPHLN1/Periphilin-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPHLN1/Periphilin-1 antibody (AB69569)

Western blot - Anti-PPHLN1/Periphilin-1 antibody (AB69569)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise