Rabbit Recombinant Monoclonal PPIC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--western-blot-img123304.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118800.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)")
![Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--western-blot-img54609.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
Alternative names
CYPC, PPIC, CYPC, Peptidyl-prolyl cis-trans isomerase C, PPIase C, Cyclophilin C, Rotamase C
Recommended products
Rabbit Recombinant Monoclonal PPIC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR15355
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250754 is the carrier-free version of Anti-PPIC antibody [EPR15355] - C-terminal ab184552.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PPIC also known as Peptidylprolyl Isomerase C functions as a molecular chaperone by catalyzing the cis-trans isomerization of proline residues in peptide chains. This activity accelerates protein folding. PPIC has a molecular mass of approximately 18 kDa. You can find PPIC expression in many tissues with significant levels in the heart and skeletal muscle. It belongs to the cyclophilin family known for its role in facilitating protein folding due to its peptidyl-prolyl cis-trans isomerase activity.
- Biological function summary
PPIC's role extends beyond simple protein folding as it forms part of a larger complex that assists in protein trafficking and assembly. It interacts with other proteins to ensure the proper folding and structural integrity of nascent peptides influencing their stability and function. The capacity to influence protein dynamics positions PPIC as an integral component of cellular homeostasis and protein quality control mechanisms.
- Pathways
PPIC assumes important roles in cellular signaling and protein trafficking pathways. Notably it participates in pathways related to immunomodulation and stress response assisting in protein folding under stress conditions. Its interaction with calcineurin a protein phosphatase indicates its involvement in the immune response and other signaling pathways mediated by calcium ions where it can impact the function of other cyclophilin family proteins.
- Associated diseases and disorders
PPIC associates with conditions like cardiac hypertrophy and ischemic diseases. Its involvement in these conditions likely connects to its role in maintaining protein homeostasis and response to cellular stress. Moreover PPIC's interaction with the calcineurin/NFAT pathway implicates it in cardiovascular diseases as this pathway participates in heart disease pathology linking to proteins such as NFAT and calmodulin. Understanding PPIC's specific contributions to these diseases could provide insights into therapeutic targets for treating related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--western-blot-img123304.jpg "Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)")
Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
This data was developed using Anti-PPIC antibody [EPR15355] - C-terminal ab184552, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (Anti-PPIC antibody [EPR15355] - C-terminal ab184552) at 1/10000 dilution
Lane 1: U-87 MG cell lysate at 20 µg
Lane 2: Human fetal kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118800.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
This data was developed using Anti-PPIC antibody [EPR15355] - C-terminal ab184552, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with Anti-PPIC antibody [EPR15355] - C-terminal ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754), expandable thumbnail](https://content.abcam.com/products/images/ppic-antibody-epr15355-bsa-and-azide-free-ab250754--western-blot-img54609.jpg "Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)")
Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (ab250754)
This data was developed using Anti-PPIC antibody [EPR15355] - C-terminal ab184552, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (Anti-PPIC antibody [EPR15355] - C-terminal ab184552) at 1/50000 dilution
All lanes: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
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