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AB250754

Anti-PPIC antibody [EPR15355] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPIC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

CYPC, PPIC, Peptidyl-prolyl cis-trans isomerase C, PPIase C, Cyclophilin C, Rotamase C

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)

This data was developed using ab184552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)
  • WB

Supplier Data

Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)

This data was developed using ab184552, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (<a href='/en-us/products/primary-antibodies/ppic-antibody-epr15355-c-terminal-ab184552'>ab184552</a>) at 1/50000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)
  • WB

Supplier Data

Western blot - Anti-PPIC antibody [EPR15355] - BSA and Azide free (AB250754)

This data was developed using ab184552, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (<a href='/en-us/products/primary-antibodies/ppic-antibody-epr15355-c-terminal-ab184552'>ab184552</a>) at 1/10000 dilution

Lane 1:

U-87 MG cell lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15355

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250754 is the carrier-free version of ab184552.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPIC also known as Peptidylprolyl Isomerase C functions as a molecular chaperone by catalyzing the cis-trans isomerization of proline residues in peptide chains. This activity accelerates protein folding. PPIC has a molecular mass of approximately 18 kDa. You can find PPIC expression in many tissues with significant levels in the heart and skeletal muscle. It belongs to the cyclophilin family known for its role in facilitating protein folding due to its peptidyl-prolyl cis-trans isomerase activity.
Biological function summary

PPIC's role extends beyond simple protein folding as it forms part of a larger complex that assists in protein trafficking and assembly. It interacts with other proteins to ensure the proper folding and structural integrity of nascent peptides influencing their stability and function. The capacity to influence protein dynamics positions PPIC as an integral component of cellular homeostasis and protein quality control mechanisms.

Pathways

PPIC assumes important roles in cellular signaling and protein trafficking pathways. Notably it participates in pathways related to immunomodulation and stress response assisting in protein folding under stress conditions. Its interaction with calcineurin a protein phosphatase indicates its involvement in the immune response and other signaling pathways mediated by calcium ions where it can impact the function of other cyclophilin family proteins.

PPIC associates with conditions like cardiac hypertrophy and ischemic diseases. Its involvement in these conditions likely connects to its role in maintaining protein homeostasis and response to cellular stress. Moreover PPIC's interaction with the calcineurin/NFAT pathway implicates it in cardiovascular diseases as this pathway participates in heart disease pathology linking to proteins such as NFAT and calmodulin. Understanding PPIC's specific contributions to these diseases could provide insights into therapeutic targets for treating related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
See full target information PPIC
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Product promise

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For full details, please see our Terms & Conditions

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