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Rabbit Recombinant Monoclonal PPIC antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552), expandable thumbnail
  • Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.

Alternative names

Peptidyl-prolyl cis-trans isomerase C, PPIase C, Cyclophilin C, Rotamase C, PPIC, CYPC

Recommended products

Rabbit Recombinant Monoclonal PPIC antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

Alternative names

Peptidyl-prolyl cis-trans isomerase C, PPIase C, Cyclophilin C, Rotamase C, PPIC, CYPC

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR15355

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein PPIC also known as Peptidylprolyl Isomerase C functions as a molecular chaperone by catalyzing the cis-trans isomerization of proline residues in peptide chains. This activity accelerates protein folding. PPIC has a molecular mass of approximately 18 kDa. You can find PPIC expression in many tissues with significant levels in the heart and skeletal muscle. It belongs to the cyclophilin family known for its role in facilitating protein folding due to its peptidyl-prolyl cis-trans isomerase activity.

Biological function summary

PPIC's role extends beyond simple protein folding as it forms part of a larger complex that assists in protein trafficking and assembly. It interacts with other proteins to ensure the proper folding and structural integrity of nascent peptides influencing their stability and function. The capacity to influence protein dynamics positions PPIC as an integral component of cellular homeostasis and protein quality control mechanisms.

Pathways

PPIC assumes important roles in cellular signaling and protein trafficking pathways. Notably it participates in pathways related to immunomodulation and stress response assisting in protein folding under stress conditions. Its interaction with calcineurin a protein phosphatase indicates its involvement in the immune response and other signaling pathways mediated by calcium ions where it can impact the function of other cyclophilin family proteins.

Associated diseases and disorders

PPIC associates with conditions like cardiac hypertrophy and ischemic diseases. Its involvement in these conditions likely connects to its role in maintaining protein homeostasis and response to cellular stress. Moreover PPIC's interaction with the calcineurin/NFAT pathway implicates it in cardiovascular diseases as this pathway participates in heart disease pathology linking to proteins such as NFAT and calmodulin. Understanding PPIC's specific contributions to these diseases could provide insights into therapeutic targets for treating related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552), expandable thumbnail

    Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552)

    All lanes: Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution

    Lane 1: U-87 MG cell lysate at 20 µg

    Lane 2: Human fetal kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552), expandable thumbnail

    Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552)

    All lanes: Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution

    All lanes: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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