Anti-PPIC antibody [EPR15355] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552)

All lanes:

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

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• WB

Supplier Data

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552)

All lanes:

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution

Lane 1:

U-87 MG cell lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

• WB

Lab

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (AB184552)

Western blot : Rabbit Monoclonal[EPR15355] to PPIC ab184552 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 25 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PPIC knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

PPIC knockout U-87 MG at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

Ramos at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false