Rabbit Recombinant Monoclonal PPIC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
sELISA | |
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Human | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
CYPC, PPIC, Peptidyl-prolyl cis-trans isomerase C, PPIase C, Cyclophilin C, Rotamase C
Rabbit Recombinant Monoclonal PPIC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
ab314567 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PPIC. The recommended pair for sandwich ELISA is:
Capture: ab314567, Human PPIC Capture Antibody (unconjugated)
Detector: Anti-PPIC antibody [EPR25823-59] - BSA and Azide free (Detector) ab314568, Human PPIC Detector Antibody (unconjugated)
The reference range value is 9.77-1250 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The protein PPIC also known as Peptidylprolyl Isomerase C functions as a molecular chaperone by catalyzing the cis-trans isomerization of proline residues in peptide chains. This activity accelerates protein folding. PPIC has a molecular mass of approximately 18 kDa. You can find PPIC expression in many tissues with significant levels in the heart and skeletal muscle. It belongs to the cyclophilin family known for its role in facilitating protein folding due to its peptidyl-prolyl cis-trans isomerase activity.
PPIC's role extends beyond simple protein folding as it forms part of a larger complex that assists in protein trafficking and assembly. It interacts with other proteins to ensure the proper folding and structural integrity of nascent peptides influencing their stability and function. The capacity to influence protein dynamics positions PPIC as an integral component of cellular homeostasis and protein quality control mechanisms.
PPIC assumes important roles in cellular signaling and protein trafficking pathways. Notably it participates in pathways related to immunomodulation and stress response assisting in protein folding under stress conditions. Its interaction with calcineurin a protein phosphatase indicates its involvement in the immune response and other signaling pathways mediated by calcium ions where it can impact the function of other cyclophilin family proteins.
PPIC associates with conditions like cardiac hypertrophy and ischemic diseases. Its involvement in these conditions likely connects to its role in maintaining protein homeostasis and response to cellular stress. Moreover PPIC's interaction with the calcineurin/NFAT pathway implicates it in cardiovascular diseases as this pathway participates in heart disease pathology linking to proteins such as NFAT and calmodulin. Understanding PPIC's specific contributions to these diseases could provide insights into therapeutic targets for treating related disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Sandwich ELISA of Human PPIC Antibody Pair - BSA and Azide free ab314566 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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