AB151246

Anti-PPIH antibody

Name: Anti-PPIH antibody (ab151246) | Abcam
Brand: Abcam Limited
SKU: AB151246

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(1 Publication)

Rabbit Polyclonal PPIH antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPIH aa 1 to C-terminus.

View Alternative Names

CYP20, CYPH, PPIH, Peptidyl-prolyl cis-trans isomerase H, PPIase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, U-snRNP-associated cyclophilin SnuCyp-20, CypH, USA-CYP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PPIH antibody (AB151246)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPIH antibody (AB151246)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PPIH with ab151246 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

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Western blot - Anti-PPIH antibody (AB151246)

12% SDS PAGE

All lanes:

Western blot - Anti-PPIH antibody (ab151246) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 19 kDa,20 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPIH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43447

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The peptidyl-prolyl cis-trans isomerase H (PPIH) also known as cyclophilin H is involved in protein folding and assembly. PPIH has a molecular weight of approximately 18 kDa. This protein expresses widely in human tissues with a notable presence in the nucleus. As an isomerase PPIH accelerates the cis-trans isomerization of prolyl bonds an important step in protein folding influencing protein conformation and function.

Biological function summary

PPIH plays a significant role in pre-mRNA splicing. It acts as a component of the spliceosome a critical molecular machinery for removing introns from pre-mRNA. Through its action within the spliceosome PPIH helps regulate the maturation of mRNA and by extension protein synthesis. Its interactions ensure the stability and functional configuration of the spliceosomal complex.

Pathways

PPIH participates in mRNA processing and splicing pathways. It engages in the spliceosomal pathway aligning closely with proteins like SMN1 and the rest of the spliceosome complex which are involved in RNA processing and gene expression regulation. The interplay of PPIH in these pathways highlights its essential role in gene expression and cellular growth functions.

Research connects PPIH with certain neurodegenerative disorders and cancer. Abnormalities in PPIH function or expression might influence diseases like spinal muscular atrophy due to its relationship with SMN1. Additionally dysregulation of PPIH-connected pathways could contribute to oncogenesis supporting its significance in cancer research. Scientists continue exploring PPIH to develop targeted therapies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed : 20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.

See full target information PPIH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 14:3733-3756 PubMed39267679

2024

PPIH as a poor prognostic factor increases cell proliferation and m6A RNA methylation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xia Chi,Peng Ye,Neng-Qi Cao,Wei-Lun Hwang,Jong-Ho Cha,Mien-Chie Hung,Kai-Wen Hsu,Xiu-Wen Yan,Wen-Hao Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPIH antibody

Immunocytochemistry/ Immunofluorescence - Anti-PPIH antibody (AB151246)

Western blot - Anti-PPIH antibody (AB151246)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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