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AB235595

Anti-PPIH antibody

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(2 Publications)

Rabbit Polyclonal PPIH antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPIH.

View Alternative Names

CYP20, CYPH, PPIH, Peptidyl-prolyl cis-trans isomerase H, PPIase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, U-snRNP-associated cyclophilin SnuCyp-20, CypH, USA-CYP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIH antibody (AB235595)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIH antibody (AB235595)

Paraffin-embedded human tonsil tissue stained for PPIH using ab235595 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PPIH antibody (AB235595)
  • WB

Supplier Data

Western blot - Anti-PPIH antibody (AB235595)

All lanes:

Western blot - Anti-PPIH antibody (ab235595) at 1/500 dilution

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/10000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPIH.

O43447

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The peptidyl-prolyl cis-trans isomerase H (PPIH) also known as cyclophilin H is involved in protein folding and assembly. PPIH has a molecular weight of approximately 18 kDa. This protein expresses widely in human tissues with a notable presence in the nucleus. As an isomerase PPIH accelerates the cis-trans isomerization of prolyl bonds an important step in protein folding influencing protein conformation and function.
Biological function summary

PPIH plays a significant role in pre-mRNA splicing. It acts as a component of the spliceosome a critical molecular machinery for removing introns from pre-mRNA. Through its action within the spliceosome PPIH helps regulate the maturation of mRNA and by extension protein synthesis. Its interactions ensure the stability and functional configuration of the spliceosomal complex.

Pathways

PPIH participates in mRNA processing and splicing pathways. It engages in the spliceosomal pathway aligning closely with proteins like SMN1 and the rest of the spliceosome complex which are involved in RNA processing and gene expression regulation. The interplay of PPIH in these pathways highlights its essential role in gene expression and cellular growth functions.

Research connects PPIH with certain neurodegenerative disorders and cancer. Abnormalities in PPIH function or expression might influence diseases like spinal muscular atrophy due to its relationship with SMN1. Additionally dysregulation of PPIH-connected pathways could contribute to oncogenesis supporting its significance in cancer research. Scientists continue exploring PPIH to develop targeted therapies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed : 20676357). Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
See full target information PPIH
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 13:e70175 PubMed40105652

2025

Tumor Cell-Expressed Herpesvirus Entry Mediator Regulates Proliferation and Adaptive Immunity in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Lu,Yijun Zhang,Wenxuan Li,Haonan Jiang,Jiapo Wang,Xiaoqing Guo

BMC cancer 24:681 PubMed38834966

2024

PPIH acts as a potential predictive biomarker for patients with common solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ye,Jianchao Ying,Haixia Chen,Zhiping Wu,Chaolin Huang,Chuan Zhang,Zhitao Chen,Haini Chen
View all publications
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Product promise

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