AB154188

Anti-PPIL1 antibody [EPR10251]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PPIL1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CYPL1, CGI-124, UNQ2425/PRO4984, PPIL1, Peptidyl-prolyl cis-trans isomerase-like 1, PPIase, Rotamase PPIL1

2 Images

Flow Cytometry (Intracellular) - Anti-PPIL1 antibody [EPR10251] (AB154188)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PPIL1 antibody [EPR10251] (AB154188)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling PPIL1 using ab154188 at 1/10 dilution (red) or rabbit IgG negative control antibody (green).

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Western blot - Anti-PPIL1 antibody [EPR10251] (AB154188)

All lanes:

Western blot - Anti-PPIL1 antibody [EPR10251] (ab154188) at 1/1000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10251

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPIL1 known as Peptidylprolyl Isomerase Like 1 is an enzyme that functions as a peptidyl-prolyl cis-trans isomerase. It facilitates protein folding by catalyzing the isomerization of proline residues in polypeptide chains. PPIL1 carries a mass of approximately 18 kDa. This protein is expressed in various tissues including the brain and immune system indicating its role in diverse cellular processes.

Biological function summary

PPIL1 contributes significantly to cellular activities by facilitating protein conformational modifications. It often operates as part of larger complexes interacting with other proteins to maintain cellular homeostasis. PPIL1 plays a vital role in response to different cellular stress conditions and its activity affects basic cellular functions especially those involving protein assembly and signaling.

Pathways

PPIL1 interacts with several critical signaling pathways influencing protein folding and cellular stress responses. It is notably involved in the protein processing pathway where it modulates the action of related proteins like Cyclophilin A (CYPA). These interactions are essential for maintaining protein homeostasis affecting processes such as cell cycle progression and apoptosis.

Abnormalities in PPIL1 expression or function have been linked to various pathological conditions such as cancer and neurodegenerative diseases. In these diseases PPIL1 may affect tumor growth or neuronal survival by interacting with proteins like p53 which is important in cancer cell apoptosis regulation. These interactions highlight PPIL1's potential as a therapeutic target for drug development and disease treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 33220177). PPIases accelerate the folding of proteins. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed : 16595688). Catalyzes prolyl peptide bond isomerization in CDC40/PRP17 (PubMed : 33220177). Plays an important role in embryonic brain development; this function is independent of its isomerase activity (PubMed : 33220177).

See full target information PPIL1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:315 PubMed39747150

2025

CDC40 suppression induces CDCA5 splicing defects and anti-proliferative effects in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Die Hu,Brigitte L Thériault,Vida Talebian,Laurent Hoffer,Julie Owen,Justin Lim,Benjamin J Blencowe,Evelyne Lima-Fernandes,Punit Saraon,Richard Marcellus,Rima Al-Awar

Cells 12: PubMed37887308

2023

IL-20 Cytokines Are Involved in the Repair of Airway Epithelial Barrier: Implication in Exposure to Cigarette Smoke and in COPD Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Barada,Sophie Salomé-Desnoulez,Fahima Madouri,Gaëtan Deslée,Christelle Coraux,Philippe Gosset,Muriel Pichavant

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPIL1 antibody [EPR10251]

Flow Cytometry (Intracellular) - Anti-PPIL1 antibody [EPR10251] (AB154188)

Western blot - Anti-PPIL1 antibody [EPR10251] (AB154188)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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