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AB249085

Anti-PPIL1 antibody [EPR10251] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPIL1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CYPL1, CGI-124, UNQ2425/PRO4984, PPIL1, Peptidyl-prolyl cis-trans isomerase-like 1, PPIase, Rotamase PPIL1

1 Images
Western blot - Anti-PPIL1 antibody [EPR10251] - BSA and Azide free (AB249085)
  • WB

Unknown

Western blot - Anti-PPIL1 antibody [EPR10251] - BSA and Azide free (AB249085)

This data was developed using ab154188, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPIL1 antibody [EPR10251] - BSA and Azide free (ab249085) at 1/1000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10251

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249085 is the carrier-free version of ab154188.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPIL1 known as Peptidylprolyl Isomerase Like 1 is an enzyme that functions as a peptidyl-prolyl cis-trans isomerase. It facilitates protein folding by catalyzing the isomerization of proline residues in polypeptide chains. PPIL1 carries a mass of approximately 18 kDa. This protein is expressed in various tissues including the brain and immune system indicating its role in diverse cellular processes.
Biological function summary

PPIL1 contributes significantly to cellular activities by facilitating protein conformational modifications. It often operates as part of larger complexes interacting with other proteins to maintain cellular homeostasis. PPIL1 plays a vital role in response to different cellular stress conditions and its activity affects basic cellular functions especially those involving protein assembly and signaling.

Pathways

PPIL1 interacts with several critical signaling pathways influencing protein folding and cellular stress responses. It is notably involved in the protein processing pathway where it modulates the action of related proteins like Cyclophilin A (CYPA). These interactions are essential for maintaining protein homeostasis affecting processes such as cell cycle progression and apoptosis.

Abnormalities in PPIL1 expression or function have been linked to various pathological conditions such as cancer and neurodegenerative diseases. In these diseases PPIL1 may affect tumor growth or neuronal survival by interacting with proteins like p53 which is important in cancer cell apoptosis regulation. These interactions highlight PPIL1's potential as a therapeutic target for drug development and disease treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 33220177). PPIases accelerate the folding of proteins. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed : 16595688). Catalyzes prolyl peptide bond isomerization in CDC40/PRP17 (PubMed : 33220177). Plays an important role in embryonic brain development; this function is independent of its isomerase activity (PubMed : 33220177).
See full target information PPIL1
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Product promise

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For full details, please see our Terms & Conditions

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