JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251870

Anti-PPIL3 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal PPIL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPIL3 aa 50 to C-terminus.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase-like 3, PPIase, Cyclophilin J, Cyclophilin-like protein PPIL3, Rotamase PPIL3, CyPJ, PPIL3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPIL3 antibody (AB251870)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPIL3 antibody (AB251870)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PPIL3 (green) using ab251870 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIL3 antibody (AB251870)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIL3 antibody (AB251870)

Paraffin-embedded human stomach, upper tissue stained for PPIL3 using ab251870 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-PPIL3 antibody (AB251870)
  • WB

Unknown

Western blot - Anti-PPIL3 antibody (AB251870)

All lanes:

Western blot - Anti-PPIL3 antibody (ab251870) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPIL3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H2H8

style
left-column

Complementary Products

Proteins & Peptides

AB113119

Recombinant human PPIL3 protein

SDS-PAGE - Recombinant human PPIL3 protein (AB113119)

  • 0
  • 0
Primary Antibodies

AB188329

Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal

RabMAb|Recombinant

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)

  • 0
  • 0
Primary Antibodies

AB181171

Anti-HNRPH2/HNRNPH2 antibody [EPR12171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (AB181171)

  • 5
  • 2
Primary Antibodies

AB283263

Anti-TPOR/MPL antibody [EPR24566-47]

20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry - Anti-TPOR/MPL antibody [EPR24566-47] (AB283263)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPIL3 or Peptidylprolyl isomerase (cyclophilin)-like 3 is an enzyme that assists in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It weighs approximately 52 kDa. PPIL3 expression can be found in various tissues including liver and thymus. The protein acts as a member of the cyclophilin family which is known for its role in protein folding and immunosuppression.
Biological function summary

The function of PPIL3 includes its contribution to the assembly of spliceosomal complexes. It interacts with other proteins to aid the splicing of pre-mRNA therefore influencing gene expression regulation. Though PPIL3 operates independently it often localizes within multiprotein complexes involved in RNA processing. Its role in the spliceosome highlights its importance in the accurate and efficient editing of RNA transcripts.

Pathways

PPIL3 participates in the RNA splicing and gene expression pathways. Within these pathways PPIL3 shows interactions with the spliceosomal proteins such as U2 small nuclear ribonucleoprotein highlighting its contribution to mRNA splicing regulation. It involves itself in adjusting the splicing machinery's fine-tuning ensuring correct mRNA processing essential for cellular functions.

PPIL3 has links to conditions such as cancer and neurodegeneration. Its role in RNA splicing means that any dysfunction resulting in spliceosomal assembly errors can contribute to diseases like cancer where incorrect mRNA splicing leads to aberrant gene expression. PPIL3 is also associated with cyclophilin B through their overlapping functions in protein folding and transport which can become impaired in neurodegenerative disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
See full target information PPIL3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e35303 PubMed39170313

2024

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Luo,Tao Chen,Junyi Deng,Ziyuan Liu,Changlong Bi,Song Lan
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com