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AB231893

Anti-PPM1A antibody

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(3 Publications)

Rabbit Polyclonal PPM1A antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPM1A.

View Alternative Names

PPPM1A, PPM1A, Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, Protein phosphatase IA, PP2C-alpha

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody (AB231893)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody (AB231893)

Paraffin-embedded human liver tissue stained for PPM1A using ab231893 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody (AB231893)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody (AB231893)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PPM1A using ab231893 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PPM1A antibody (AB231893)
  • WB

Supplier Data

Western blot - Anti-PPM1A antibody (AB231893)

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Western blot - Anti-PPM1A antibody (ab231893) at 2 µg/mL

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Recombinant human PPM1A protein

Predicted band size: 42 kDa

false

Western blot - Anti-PPM1A antibody (AB231893)
  • WB

Supplier Data

Western blot - Anti-PPM1A antibody (AB231893)

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Western blot - Anti-PPM1A antibody (ab231893) at 2 µg/mL

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HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 42 kDa

false

Western blot - Anti-PPM1A antibody (AB231893)
  • WB

Supplier Data

Western blot - Anti-PPM1A antibody (AB231893)

All lanes:

Western blot - Anti-PPM1A antibody (ab231893) at 2 µg/mL

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Pig liver lysate

Predicted band size: 42 kDa

false

Western blot - Anti-PPM1A antibody (AB231893)
  • WB

Supplier Data

Western blot - Anti-PPM1A antibody (AB231893)

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Western blot - Anti-PPM1A antibody (ab231893) at 2 µg/mL

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HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 42 kDa

false

Western blot - Anti-PPM1A antibody (AB231893)
  • WB

Supplier Data

Western blot - Anti-PPM1A antibody (AB231893)

All lanes:

Western blot - Anti-PPM1A antibody (ab231893) at 2 µg/mL

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Pig heart lysate

Predicted band size: 42 kDa,58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPM1A.

P35813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231893 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1A also known as protein phosphatase 1A or PP2C-alpha functions as a serine/threonine phosphatase. This protein plays an important role in removing phosphate groups from serine and threonine residues on proteins. The molecular mass of PPM1A is approximately 42-44 kDa. Expression of PPM1A is widespread and found in various human tissues indicating its significant involvement in multiple cellular processes.
Biological function summary

PPM1A regulates many cellular mechanisms by dephosphorylating targets and acting as a negative regulator of several signaling pathways. It modulates stress responses cell cycle progression and immune responses. PPM1A does not typically form complexes with other proteins but interacts closely with specific signaling molecules to control their activity. This regulation influences cellular homeostasis and growth in response to external and internal signals.

Pathways

PPM1A influences the TGF-beta signaling pathway where it interacts with and deactivates SMAD proteins by dephosphorylation. It also participates in the p38 MAPK pathway. These interactions highlight its role in controlling transduction signals initiated by growth factors and stress. By regulating these pathways PPM1A maintains a balance in cellular responses to growth and environmental stresses.

PPM1A links to cancer and inflammatory diseases. Its ability to modulate phosphorylation states impacts cell proliferation and immune responses critical factors in these conditions. For instance PPM1A interaction with SMAD3 in the TGF-beta pathway can influence tumor progression. Additionally its regulation of proteins such as p38 MAPK can alter inflammatory responses affecting inflammatory disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
See full target information PPM1A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

Molecular medicine reports 26: PubMed36069235

2022

Platyconic acid A‑induced PPM1A upregulation inhibits the proliferation, inflammation and extracellular matrix deposition of TGF‑β1‑induced lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Su,Yanfen Tang,Cong Wang,Haifeng Huang,Haihui Hou

Cells 9: PubMed32727081

2020

Protamine-2 Deficiency Initiates a Reactive Oxygen Species (ROS)-Mediated Destruction Cascade during Epididymal Sperm Maturation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Schneider,Farhad Shakeri,Christian Trötschel,Lena Arévalo,Alexander Kruse,Andreas Buness,Ansgar Poetsch,Klaus Steger,Hubert Schorle
View all publications
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