Rabbit Polyclonal PPM1D/WIP1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.
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- Oncology letters 25:312023Wip1 contributes to the adaptation of HepG2 human liver cancer cells to stress hormone-induced DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaoxiang Li,Yazhi Qian,Yuzhu Chen,Mingyue Cao,Xiaozhou Yang,Dexin Kong,Guiping Wang,Haiyan An,Nan Yang,Wei Huang,Yanyong LiuPubMed 36589663
- Molecular cell 81:1292-1308.e112021Systematic characterization of mutations altering protein degradation in human cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCollin Tokheim,Xiaoqing Wang,Richard T Timms,Boning Zhang,Elijah L Mena,Binbin Wang,Cynthia Chen,Jun Ge,Jun Chu,Wubing Zhang,Stephen J Elledge,Myles Brown,X Shirley LiuPubMed 33567269
- Nature communications 11:48752020Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianlin Liu et. al.PubMed 32978388
- Signal transduction and targeted therapy 5:362020WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiyuan Deng et. al.PubMed 32296033
- Oncology reports 43:783-7942020Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong-Wu Lu et. al.PubMed 31922231
- Journal of cellular biochemistry 120:15709-157182019Wip1 cooperates with KPNA2 to modulate the cell proliferation and migration of colorectal cancer via a p53-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Wang et. al.PubMed 31127650
- EBioMedicine 44:138-1492019APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyuan Gong et. al.PubMed 31105033
- Journal of cellular biochemistry 120:5652-56652018P65-mediated miR-590 inhibition modulates the chemoresistance of osteosarcoma to doxorubicin through targeting wild-type p53-induced phosphatase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Long et. al.PubMed 30387173
- Oncology letters 13:875-8802017Wild-type p53-induced phosphatase 1 is a prognostic marker and therapeutic target in bladder transitional cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Peng Wang,Shu-Yuan Chen,Ye TianPubMed 28356972
- Molecular biology reports 40:5013-262013Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the replicative potential of cancer cells treated with a genotoxic dose of resveratrol.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Xenopus laevis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
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2 products for Alternative Product
Target data
Function
Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).
Alternative names
WIP1, PPM1D, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PP2C-delta
Recommended products
Rabbit Polyclonal PPM1D/WIP1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PPM1D protein also known as wild-type p53-induced phosphatase 1 (WIP1) is a serine/threonine phosphatase. Its molecular weight is approximately 66 kDa. PPM1D is an enzyme expressed in various tissues including the brain heart and kidney. This phosphatase plays an important role in the regulation of the cellular stress response by dephosphorylating specific substrates.
- Biological function summary
PPM1D/WIP1 acts as a modulator of cellular stress and survival mechanisms. The protein influences the DNA damage response and supports cell cycle progression by deactivating critical checkpoint proteins such as p38 MAPK and p53. It does not form part of a larger protein complex but interacts directly with these proteins to exert its biological functions.
- Pathways
PPM1D engages with mechanisms linked to the DNA damage response and the p53 signaling pathway. It counteracts the activation of p53 a protein integral in cell cycle arrest and apoptosis which helps cells to recover post-DNA damage. PPM1D also interacts with proteins like ATM and CHK2 which are part of the DNA repair pathway playing a role in cellular recovery and survival.
- Associated diseases and disorders
PPM1D links to cancer development and progression. Its role in deactivating p53 and facilitating continued cell proliferation makes it relevant to tumorigenesis. Also PPM1D's connection to ATM and the DNA damage response becomes significant in neurodegenerative diseases. Dysregulation of PPM1D can contribute to an improper cellular response to genomic instability contributing to disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PPM1D/WIP1 antibody (ab31270)
All lanes: Western blot - Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 20min
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