AB31270

Anti-PPM1D/WIP1 antibody

Name: Anti-PPM1D/WIP1 antibody (ab31270) | Abcam
Brand: Abcam Limited
SKU: AB31270

(1 Review)

(11 Publications)

Rabbit Polyclonal PPM1D/WIP1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

WIP1, PPM1D, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PP2C-delta

1 Images

Unknown

Western blot - Anti-PPM1D/WIP1 antibody (AB31270)

All lanes:

Western blot - Anti-PPM1D/WIP1 antibody (ab31270) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPM1D protein also known as wild-type p53-induced phosphatase 1 (WIP1) is a serine/threonine phosphatase. Its molecular weight is approximately 66 kDa. PPM1D is an enzyme expressed in various tissues including the brain heart and kidney. This phosphatase plays an important role in the regulation of the cellular stress response by dephosphorylating specific substrates.

Biological function summary

PPM1D/WIP1 acts as a modulator of cellular stress and survival mechanisms. The protein influences the DNA damage response and supports cell cycle progression by deactivating critical checkpoint proteins such as p38 MAPK and p53. It does not form part of a larger protein complex but interacts directly with these proteins to exert its biological functions.

Pathways

PPM1D engages with mechanisms linked to the DNA damage response and the p53 signaling pathway. It counteracts the activation of p53 a protein integral in cell cycle arrest and apoptosis which helps cells to recover post-DNA damage. PPM1D also interacts with proteins like ATM and CHK2 which are part of the DNA repair pathway playing a role in cellular recovery and survival.

PPM1D links to cancer development and progression. Its role in deactivating p53 and facilitating continued cell proliferation makes it relevant to tumorigenesis. Also PPM1D's connection to ATM and the DNA damage response becomes significant in neurodegenerative diseases. Dysregulation of PPM1D can contribute to an improper cellular response to genomic instability contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the negative regulation of p53 expression (PubMed : 23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed : 15870257, PubMed : 16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed : 21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).

See full target information PPM1D

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 25:31 PubMed36589663

2023

Wip1 contributes to the adaptation of HepG2 human liver cancer cells to stress hormone-induced DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Gaoxiang Li,Yazhi Qian,Yuzhu Chen,Mingyue Cao,Xiaozhou Yang,Dexin Kong,Guiping Wang,Haiyan An,Nan Yang,Wei Huang,Yanyong Liu

Molecular cell 81:1292-1308.e11 PubMed33567269

2021

Systematic characterization of mutations altering protein degradation in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Collin Tokheim,Xiaoqing Wang,Richard T Timms,Boning Zhang,Elijah L Mena,Binbin Wang,Cynthia Chen,Jun Ge,Jun Chu,Wubing Zhang,Stephen J Elledge,Myles Brown,X Shirley Liu

Nature communications 11:4875 PubMed32978388

2020

Characterization of BRCA1-deficient premalignant tissues and cancers identifies Plekha5 as a tumor metastasis suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Jianlin Liu,Ragini Adhav,Kai Miao,Sek Man Su,Lihua Mo,Un In Chan,Xin Zhang,Jun Xu,Jianjie Li,Xiaodong Shu,Jianming Zeng,Xu Zhang,Xueying Lyu,Lakhansing Pardeshi,Kaeling Tan,Heng Sun,Koon Ho Wong,Chuxia Deng,Xiaoling Xu

Signal transduction and targeted therapy 5:36 PubMed32296033

2020

WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyuan Deng,Liang Liu,Xiaoming Tan,Zhen Zhang,Jianjun Li,Yang Ou,Xin Wang,Shuang Yang,Rong Xiang,Peiqing Sun

Oncology reports 43:783-794 PubMed31922231

2020

Silencing of PPM1D inhibits cell proliferation and invasion through the p38 MAPK and p53 signaling pathway in papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Wu Lu,Duo Wen,Wen-Jun Wei,Li-Tao Han,Jun Xiang,Yu-Long Wang,Yu Wang,Tian Liao,Qing-Hai Ji

Journal of cellular biochemistry 120:15709-15718 PubMed31127650

2019

Wip1 cooperates with KPNA2 to modulate the cell proliferation and migration of colorectal cancer via a p53-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Yahui Zhao,Kuijie Liu,Xianghe Liu,Jianwei Liang,Haitao Zhou,Zheng Wang,Zhixiang Zhou,Ningzhi Xu

EBioMedicine 44:138-149 PubMed31105033

2019

APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Gong,Fei Liu,Xiaoyu Liu,Shengping Min,Nan Wu,Xincheng Liu,Yueguang Liu,Sue Han,Yijie Zhang,Yuefang Zhang,Yudong Hu,Xuegang Liu,Xiaojing Wang

Journal of cellular biochemistry 120:5652-5665 PubMed30387173

2018

P65-mediated miR-590 inhibition modulates the chemoresistance of osteosarcoma to doxorubicin through targeting wild-type p53-induced phosphatase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Long,Xiang-Jin Lin

Oncology letters 13:875-880 PubMed28356972

2017

Wild-type p53-induced phosphatase 1 is a prognostic marker and therapeutic target in bladder transitional cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Peng Wang,Shu-Yuan Chen,Ye Tian

Molecular biology reports 40:5013-26 PubMed23666059

2013

Nutlin-3a, an MDM2 antagonist and p53 activator, helps to preserve the replicative potential of cancer cells treated with a genotoxic dose of resveratrol.

Applications

Species

Human

Artur Zajkowicz,Małgorzata Krześniak,Iwona Matuszczyk,Magdalena Głowala-Kosińska,Dorota Butkiewicz,Marek Rusin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PPM1D/WIP1 antibody

Western blot - Anti-PPM1D/WIP1 antibody (AB31270)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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