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Rabbit Recombinant Monoclonal PPM1D/WIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.


Images

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439), expandable thumbnail
  • Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PFlow CytIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).

Alternative names

WIP1, PPM1D, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PP2C-delta

Recommended products

Rabbit Recombinant Monoclonal PPM1D/WIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22960-39
Purification technique
Affinity purification Protein A
Specificity
The antibody detects specifically PPM1D430.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The PPM1D protein also known as wild-type p53-induced phosphatase 1 (WIP1) is a serine/threonine phosphatase. Its molecular weight is approximately 66 kDa. PPM1D is an enzyme expressed in various tissues including the brain heart and kidney. This phosphatase plays an important role in the regulation of the cellular stress response by dephosphorylating specific substrates.

Biological function summary

PPM1D/WIP1 acts as a modulator of cellular stress and survival mechanisms. The protein influences the DNA damage response and supports cell cycle progression by deactivating critical checkpoint proteins such as p38 MAPK and p53. It does not form part of a larger protein complex but interacts directly with these proteins to exert its biological functions.

Pathways

PPM1D engages with mechanisms linked to the DNA damage response and the p53 signaling pathway. It counteracts the activation of p53 a protein integral in cell cycle arrest and apoptosis which helps cells to recover post-DNA damage. PPM1D also interacts with proteins like ATM and CHK2 which are part of the DNA repair pathway playing a role in cellular recovery and survival.

Associated diseases and disorders

PPM1D links to cancer development and progression. Its role in deactivating p53 and facilitating continued cell proliferation makes it relevant to tumorigenesis. Also PPM1D's connection to ATM and the DNA damage response becomes significant in neurodegenerative diseases. Dysregulation of PPM1D can contribute to an improper cellular response to genomic instability contributing to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lanes 1-4: 70 seconds; Lanes 5-8: 48 seconds.

    All lanes: Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

    Lane 5: HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

    Lane 6: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

    Lane 7: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 8: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT-116 (Human colorectal carcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HCT-116 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439), expandable thumbnail

    Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 3 minutes

    All lanes: Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human lung cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 67 kDa

    Observed band size: 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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