AB234439

Anti-PPM1D/WIP1 antibody [EPR22960-39]

Name: Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) | Abcam
Brand: Abcam Limited
SKU: AB234439
Price: 570 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PPM1D/WIP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

WIP1, PPM1D, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PP2C-delta

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT-116 (Human colorectal carcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HCT-116 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Lab

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1-4 : 70 seconds; Lanes 5-8 : 48 seconds.

All lanes:

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

Lane 5:

HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 6:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 8:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 67 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 minutes

All lanes:

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human lung cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Blocking buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 67 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22960-39

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody detects specifically PPM1D430.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPM1D protein also known as wild-type p53-induced phosphatase 1 (WIP1) is a serine/threonine phosphatase. Its molecular weight is approximately 66 kDa. PPM1D is an enzyme expressed in various tissues including the brain heart and kidney. This phosphatase plays an important role in the regulation of the cellular stress response by dephosphorylating specific substrates.

Biological function summary

PPM1D/WIP1 acts as a modulator of cellular stress and survival mechanisms. The protein influences the DNA damage response and supports cell cycle progression by deactivating critical checkpoint proteins such as p38 MAPK and p53. It does not form part of a larger protein complex but interacts directly with these proteins to exert its biological functions.

Pathways

PPM1D engages with mechanisms linked to the DNA damage response and the p53 signaling pathway. It counteracts the activation of p53 a protein integral in cell cycle arrest and apoptosis which helps cells to recover post-DNA damage. PPM1D also interacts with proteins like ATM and CHK2 which are part of the DNA repair pathway playing a role in cellular recovery and survival.

PPM1D links to cancer development and progression. Its role in deactivating p53 and facilitating continued cell proliferation makes it relevant to tumorigenesis. Also PPM1D's connection to ATM and the DNA damage response becomes significant in neurodegenerative diseases. Dysregulation of PPM1D can contribute to an improper cellular response to genomic instability contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the negative regulation of p53 expression (PubMed : 23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed : 15870257, PubMed : 16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed : 21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).

See full target information PPM1D

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPM1D/WIP1 antibody [EPR22960-39]

Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Western blot - Anti-PPM1D/WIP1 antibody [EPR22960-39] (AB234439)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise