AB137122

Anti-PPM1E antibody [EPR8721]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal PPM1E antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CAMKN, KIAA1072, POPX1, PPM1E, Protein phosphatase 1E, Ca(2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-nucleus, Partner of PIX 1, Partner of PIX-alpha, CaMKP-N, CaMKN, Partner of PIXA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (AB137122)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (AB137122)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-PPM1E antibody [EPR8721] (AB137122)

All lanes:

Western blot - Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Lane 3:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

Observed band size: 84 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8721

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1E also known as protein phosphatase M or POPX1 is a serine/threonine phosphatase with a molecular mass of approximately 82 kDa. It is expressed in various tissues including the brain muscle and heart. PPM1E dephosphorylates its substrates thereby regulating their activities. The enzyme negatively affects phosphorylation-dependent signaling pathways which impacts several biological processes. Its wide expression pattern indicates a broad role in cellular function.

Biological function summary

PPM1E associates with certain complexes that regulate cytoskeletal dynamics and cellular stress responses. It modulates proteins involved in actin cytoskeleton reorganization influencing cell motility and morphology. PPM1E interacts with and regulates targets like CaMKII and PAK which are essential for cell signaling and homeostasis. By acting on these targets PPM1E maintains balance in various cellular activities and processes.

Pathways

PPM1E regulates critical signaling pathways such as the MAPK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK pathway PPM1E regulates signal transduction impacting cellular responses to proliferative signals. It also interacts with calcium/calmodulin-dependent protein kinase modulating calcium signaling which is important for maintaining cardiovascular and neuronal functions. These pathways highlight PPM1E's importance in cellular signaling networks and its interactions with other proteins like MEK and CaMKII.

PPM1E links to neurological disorders and cancer. Changes in PPM1E expression or activity are associated with Alzheimer's disease with its regulatory function influencing proteins like tau an important player in neurodegeneration. In cancer altered PPM1E expression affects cell proliferation and survival often interacting with oncogenic proteins such as RAS. These associations underline the potential of PPM1E as a target for therapeutic strategies in diverse disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2 (By similarity).

See full target information PPM1E

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105695 PubMed38301894

2024

The BHLHE40‒PPM1F‒AMPK pathway regulates energy metabolism and is associated with the aggressiveness of endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuo Asanoma,Hiroshi Yagi,Ichiro Onoyama,Lin Cui,Emiko Hori,Minoru Kawakami,Shoji Maenohara,Kazuhisa Hachisuga,Hiroshi Tomonobe,Keisuke Kodama,Masafumi Yasunaga,Tatsuhiro Ohgami,Kaoru Okugawa,Hideaki Yahata,Hiroyuki Kitao,Kiyoko Kato

Viruses 15: PubMed37766305

2023

The Gene Transcriptional Activation and Protein Degradation Mediated by Transactivator Tas of Prototype Foamy Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Ma,Jie Wei,Jing Song,Zhongxiang Hu,Ruifen Zhang,Zhi Li,Yan Sun

Virulence 13:342-354 PubMed35132916

2022

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Jie,Zhang Rui-Fen,Hu Zhong-Xiang,Wu Yan,Liu Wei-Na,Ma Yong-Ping,Song Jing,Chen Jing-Yi,Liu Wan-Hong,He Xiao-Hua,Li Zhi,Sun Yan

Cancer cell 32:624-638.e5 PubMed29056426

2017

mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Valvezan,Marc Turner,Amine Belaid,Hilaire C Lam,Spencer K Miller,Molly C McNamara,Christian Baglini,Benjamin E Housden,Norbert Perrimon,David J Kwiatkowski,John M Asara,Elizabeth P Henske,Brendan D Manning

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPM1E antibody [EPR8721]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (AB137122)

Western blot - Anti-PPM1E antibody [EPR8721] (AB137122)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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