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Rabbit Polyclonal PPM1F/POPX2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein phosphatase 1F.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394), expandable thumbnail
  • Western blot - Anti-PPM1F/POPX2 antibody (AB200394), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Protein phosphatase 1F. The exact immunogen used to generate this antibody is proprietary information. Database link P49593

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Alternative names

Recommended products

Rabbit Polyclonal PPM1F/POPX2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein phosphatase 1F.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Protein phosphatase 1F. The exact immunogen used to generate this antibody is proprietary information. Database link P49593
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PPM1F also known as POPX2 is a protein phosphatase that belongs to the PP2C family. It functions as a serine/threonine phosphatase and has a molecular mass of approximately 48 kDa. PPM1F is expressed across various tissues including the brain muscle and liver. It plays a role in dephosphorylating proteins involved in numerous cellular processes. By regulating phosphorylation status PPM1F impacts cellular activities such as cell cycle progress and apoptotic pathways.

Biological function summary

This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.

Pathways

Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.

Associated diseases and disorders

Alterations in PPM1F function have correlations with cancer and neurodegenerative diseases. In cancer PPM1F's dephosphorylation roles impact pathways relevant to cell proliferation and apoptosis contributing to tumor progression. Abnormal regulation of PPM1F has also been observed in certain neurodegenerative disorders where its interactions with proteins such as MAPKs affect neuronal survival and distress responses. The link between PPM1F and these conditions highlights its potential as a therapeutic target in disease management.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (ab200394), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (ab200394)

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PPM1F/POPX2 with ab200394 at 1/40 dilution.

  • Western blot - Anti-PPM1F/POPX2 antibody (ab200394), expandable thumbnail

    Western blot - Anti-PPM1F/POPX2 antibody (ab200394)

    Gel: 8% SDS-PAGE.

    All lanes: Western blot - Anti-PPM1F/POPX2 antibody (ab200394) at 1/200 dilution

    Lane 1: K562 cell lysate at 40 µg

    Lane 2: Human fetal liver tissue lysate at 40 µg

    Predicted band size: 50 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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