AB200394

Anti-PPM1F/POPX2 antibody

Name: Anti-PPM1F/POPX2 antibody (ab200394) | Abcam
Brand: Abcam Limited
SKU: AB200394

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(2 Publications)

Rabbit Polyclonal PPM1F/POPX2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPM1F.

View Alternative Names

KIAA0015, POPX2, PPM1F, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase, hFem-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PPM1F/POPX2 with ab200394 at 1/40 dilution.

Supplier Data

Western blot - Anti-PPM1F/POPX2 antibody (AB200394)

Gel : 8% SDS-PAGE.

All lanes:

Western blot - Anti-PPM1F/POPX2 antibody (ab200394) at 1/200 dilution

Lane 1:

K562 cell lysate at 40 µg

Lane 2:

Human fetal liver tissue lysate at 40 µg

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PPM1F. The exact immunogen used to generate this antibody is proprietary information.

P49593

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1F also known as POPX2 is a protein phosphatase that belongs to the PP2C family. It functions as a serine/threonine phosphatase and has a molecular mass of approximately 48 kDa. PPM1F is expressed across various tissues including the brain muscle and liver. It plays a role in dephosphorylating proteins involved in numerous cellular processes. By regulating phosphorylation status PPM1F impacts cellular activities such as cell cycle progress and apoptotic pathways.

Biological function summary

This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.

Pathways

Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.

Alterations in PPM1F function have correlations with cancer and neurodegenerative diseases. In cancer PPM1F's dephosphorylation roles impact pathways relevant to cell proliferation and apoptosis contributing to tumor progression. Abnormal regulation of PPM1F has also been observed in certain neurodegenerative disorders where its interactions with proteins such as MAPKs affect neuronal survival and distress responses. The link between PPM1F and these conditions highlights its potential as a therapeutic target in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

See full target information PPM1F

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105695 PubMed38301894

2024

The BHLHE40‒PPM1F‒AMPK pathway regulates energy metabolism and is associated with the aggressiveness of endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuo Asanoma,Hiroshi Yagi,Ichiro Onoyama,Lin Cui,Emiko Hori,Minoru Kawakami,Shoji Maenohara,Kazuhisa Hachisuga,Hiroshi Tomonobe,Keisuke Kodama,Masafumi Yasunaga,Tatsuhiro Ohgami,Kaoru Okugawa,Hideaki Yahata,Hiroyuki Kitao,Kiyoko Kato

Frontiers in genetics 11:582796 PubMed33193710

2020

Gene and Protein Expression in Subjects With a Nystagmus-Associated AHR Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Borovok,Celeste Weiss,Rajech Sharkia,Michal Reichenstein,Bernd Wissinger,Abdussalam Azem,Muhammad Mahajnah

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPM1F/POPX2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1F/POPX2 antibody (AB200394)

Western blot - Anti-PPM1F/POPX2 antibody (AB200394)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise