Rabbit Polyclonal PPM1G antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PPM1G.
View Alternative Names
PPM1C, PPM1G, Protein phosphatase 1G, Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (AB70794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PPM1G with ab70794 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-PPM1G antibody (AB70794)
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70794 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection : Chemiluminescence with an exposure time of 1 second.
All lanes:
Immunoprecipitation - Anti-PPM1G antibody (ab70794)
Predicted band size: 59 kDa
false
- WB
Unknown
Western blot - Anti-PPM1G antibody (AB70794)
All lanes:
Western blot - Anti-PPM1G antibody (ab70794) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HeLa whole cell lysate at 5 µg
Lane 4:
293T whole cell lysate at 50 µg
Predicted band size: 59 kDa
Observed band size: 130 kDa,70 kDa
true
Exposure time: 10s
- WB
CiteAb
Western blot - Anti-PPM1G antibody (AB70794)
PPM1G western blot using anti-PPM1G antibody ab70794. Publication image and figure legend from Pyo, J., Ryu, J., et al., 2018, Int J Mol Sci, PubMed 30081604.
ab70794 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70794 please see the product overview.
Protein phosphatase 1 gamma (PPM1G) downregulates hypoxia-inducible factor (HIF)-1α expression under normoxic conditions. HEK293T cells were transfected with MycHis-Empty (-) or MycHis-PPM1G (+) (A), control siRNA (-) or PPM1G-targeting siRNA (siPPM1G) (B), vectors harboring HIF-1α fused with various tags (C), and vectors encoding other proteins (D). Cells were then cultured under normoxic conditions. Expression of each protein was determined by Western blot and PPM1G knockdown was confirmed by RT-PCR. (A) FLAG-HIF-1α expression in cells transfected with various amounts of the PPM1G vector; (B) Expressions of FLAG-HIF-1α protein and PPM1G mRNA in PPM1G-depleted cells; (C) FLAG-HIF-1α, HA-HIF-1α, GST-HIF-1α, and GFP-HIF-1α expression in PPM1G-overexpressing cells. Arrows indicate tagged HIF-1α; (D) FLAG-HIF-1α, FLAG-DBC1, and FLAG-PCAF expression in PPM1G-overexpressing cells; (E) HEK293T cells were co-transfected with the PGK1-HRE-Luc reporter, a β-galactosidase-encoding plasmid and either MycHis-Empty (-) or MycHis-PPM1G (+) in the presence of HIF-1α overexpression. Relative luciferase activity was calculated after normalization of transfection efficiency according to the β-galactosidase activity. * p < 0.05; significantly different from only FLAG-HIF-1α-transfected cells.
false
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Reactivity data
Properties and storage information
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Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPM1G contributes to several cellular functions by interacting with different substrates. As a component of the cellular machinery it is involved in processes such as RNA processing and regulation of mitosis. The enzyme participates in dephosphorylation events critical for the assembly and disassembly of the spliceosome complex which is important for accurate mRNA splicing. PPM1G's activity helps maintain the proper function of these cellular processes ensuring the stability and output of gene expression.
Pathways
Several significant cellular activities are influenced by PPM1G's regulatory mechanisms. It is implicated in the mitogen-activated protein kinase (MAPK) signaling pathway and the cell cycle regulation pathway. These pathways are vital for controlling cell growth and division. In these contexts PPM1G interacts with various other proteins such as p53 and cyclin-dependent kinases (CDKs) linking it to cell cycle checkpoints and stress responses. These interactions allow PPM1G to regulate and coordinate cellular responses to external and internal signals.
Product protocols
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Target data
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
iScience 27:109116 PubMed38384839
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 19: PubMed30081604
2018
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 5:1256-68 PubMed24316072
2013
Applications
ChIP
Species
Human
Biochimica et biophysica acta 1823:1092-101 PubMed22504172
2012
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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