AB184184

Anti-PPM1G antibody [EPR13362]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PPM1G antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PPM1C, PPM1G, Protein phosphatase 1G, Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma

2 Images

Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362] (AB184184)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362] (AB184184)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab184184 at 1/250 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-PPM1G antibody [EPR13362] (AB184184)

All lanes:

Western blot - Anti-PPM1G antibody [EPR13362] (ab184184) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 59 kDa

Observed band size: 75 kDa

false

Carrier free

Anti-PPM1G antibody [EPR13362] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13362

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1G also known as protein phosphatase 1G is a member of the protein phosphatase 2C (PP2C) family. This enzyme has a molecular mass of approximately 58 kDa. It localizes mainly in the nucleus and is expressed in various tissues including the brain testis and skeletal muscle. PPM1G functions as a serine/threonine phosphatase removing phosphate groups from target proteins and playing a role in the regulation of cellular stress responses and cell cycle progression.

Biological function summary

PPM1G contributes to several cellular functions by interacting with different substrates. As a component of the cellular machinery it is involved in processes such as RNA processing and regulation of mitosis. The enzyme participates in dephosphorylation events critical for the assembly and disassembly of the spliceosome complex which is important for accurate mRNA splicing. PPM1G's activity helps maintain the proper function of these cellular processes ensuring the stability and output of gene expression.

Pathways

Several significant cellular activities are influenced by PPM1G's regulatory mechanisms. It is implicated in the mitogen-activated protein kinase (MAPK) signaling pathway and the cell cycle regulation pathway. These pathways are vital for controlling cell growth and division. In these contexts PPM1G interacts with various other proteins such as p53 and cyclin-dependent kinases (CDKs) linking it to cell cycle checkpoints and stress responses. These interactions allow PPM1G to regulate and coordinate cellular responses to external and internal signals.

PPM1G has connections with illnesses like cancer and neurodegenerative diseases. In cancer its role in cell cycle regulation can affect tumor growth and progression often depending on its interactions with proteins like CDK. In neurodegenerative disorders PPM1G might play a part due to its involvement in RNA processing and protein dephosphorylation processes which are critical for neuron function. Any dysregulation in these interactions may contribute to disease pathology making PPM1G a point of interest for understanding and potentially treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PPM1G

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 291:4714-4731 PubMed39206622

2024

PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglan Hao,Guiqin Han,Xin Liang,Yongtong Ruan,Chen Huang,Naer Sa,Hang Hu,Bixi Hu,Zhongqi Li,Kai Zhang,Ping Gao,Xiaoming Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPM1G antibody [EPR13362]

Flow Cytometry (Intracellular) - Anti-PPM1G antibody [EPR13362] (AB184184)

Western blot - Anti-PPM1G antibody [EPR13362] (AB184184)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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