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AB170412

Anti-PPOX antibody [EPR10400]

1

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal PPOX antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Protoporphyrinogen oxidase, PPO, PPOX

3 Images
Flow Cytometry (Intracellular) - Anti-PPOX antibody [EPR10400] (AB170412)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPOX antibody [EPR10400] (AB170412)

Intracellular flow cytometric analysis of permeabilized HT-1080 cells usingab170412 (red) at a 1/100 dilutionor a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-PPOX antibody [EPR10400] (AB170412)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPOX antibody [EPR10400] (AB170412)

Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling PPOX with ab170412 at a 1/100 dilution (green). DAPI nuclear staining (blue).

Western blot - Anti-PPOX antibody [EPR10400] (AB170412)
  • WB

Supplier Data

Western blot - Anti-PPOX antibody [EPR10400] (AB170412)

All lanes:

Western blot - Anti-PPOX antibody [EPR10400] (ab170412) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10400

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPOX also known as protoporphyrinogen oxidase is an important enzyme in heme biosynthesis. It catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX. The mass of PPOX is about 50 kDa and it is expressed in the mitochondria of cells across various tissues including the liver and bone marrow. PPOX's activity is important for producing heme which is an important component of hemoglobin.
Biological function summary

The enzyme PPOX contributes to heme production working within the mitochondrial membrane. It is part of the heme biosynthetic pathway and functions in converting protoporphyrinogen IX the last precursor to heme. This enzymatic action is important for producing heme groups required for hemoglobin myoglobin cytochromes and other heme-containing proteins. Without PPOX cells could not efficiently synthesize these important proteins.

Pathways

PPOX plays a role in the protoporphyrin IX biosynthesis pathway which forms part of the larger heme biosynthesis pathway. It interacts with other enzymes like coproporphyrinogen oxidase (CPOX) and ferrochelatase (FECH) which work sequentially in the pathway. These interactions ensure the completed heme molecule for functions like oxygen transport and electron transfer.

Defects in PPOX are linked to variegate porphyria a type of porphyria causing skin and neurological symptoms. Mutations in the PPOX gene reduce enzyme activity leading to the accumulation of protoporphyrinogen IX. This disease connects PPOX with other enzymes in the heme pathway such as hydroxymethylbilane synthase (HMBS) which is involved in acute intermittent porphyria. Proper understanding of PPOX and its pathway interactions helps in managing and diagnosing such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
See full target information PPOX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 28:573-587.e13 PubMed30017355

2018

Serine Synthesis via PHGDH Is Essential for Heme Production in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Saar Vandekeere,Charlotte Dubois,Joanna Kalucka,Mark R Sullivan,Melissa García-Caballero,Jermaine Goveia,Rongyuan Chen,Frances F Diehl,Libat Bar-Lev,Joris Souffreau,Andreas Pircher,Saran Kumar,Stefan Vinckier,Yoshio Hirabayashi,Shigeki Furuya,Luc Schoonjans,Guy Eelen,Bart Ghesquière,Eli Keshet,Xuri Li,Matthew G Vander Heiden,Mieke Dewerchin,Peter Carmeliet
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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