AB249564

Anti-PPOX antibody [EPR10401] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PPOX antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Protoporphyrinogen oxidase, PPO, PPOX

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PPOX using ab170884 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

ab170884 showing +ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

ab170884 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-PPOX antibody [EPR10401] (<a href='/en-us/products/primary-antibodies/ppox-antibody-epr10401-ab170884'>ab170884</a>) at 1/1000 dilution

Lanes 2 - 4:

Anti-cardiac Troponin I antibody (<a href='/en-us/products/unavailable/cardiac-troponin-i-antibody-ab1000'>ab1000</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HT1080 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 51 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

ab170884 showing +ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

This data was developed using ab170884, the same antibody clone in a different buffer formulation.

ab170884 showing +ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unconjugated

Anti-PPOX antibody [EPR10401]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10401

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249564 is the carrier-free version of ab170884.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPOX also known as protoporphyrinogen oxidase is an important enzyme in heme biosynthesis. It catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX. The mass of PPOX is about 50 kDa and it is expressed in the mitochondria of cells across various tissues including the liver and bone marrow. PPOX's activity is important for producing heme which is an important component of hemoglobin.

Biological function summary

The enzyme PPOX contributes to heme production working within the mitochondrial membrane. It is part of the heme biosynthetic pathway and functions in converting protoporphyrinogen IX the last precursor to heme. This enzymatic action is important for producing heme groups required for hemoglobin myoglobin cytochromes and other heme-containing proteins. Without PPOX cells could not efficiently synthesize these important proteins.

Pathways

PPOX plays a role in the protoporphyrin IX biosynthesis pathway which forms part of the larger heme biosynthesis pathway. It interacts with other enzymes like coproporphyrinogen oxidase (CPOX) and ferrochelatase (FECH) which work sequentially in the pathway. These interactions ensure the completed heme molecule for functions like oxygen transport and electron transfer.

Defects in PPOX are linked to variegate porphyria a type of porphyria causing skin and neurological symptoms. Mutations in the PPOX gene reduce enzyme activity leading to the accumulation of protoporphyrinogen IX. This disease connects PPOX with other enzymes in the heme pathway such as hydroxymethylbilane synthase (HMBS) which is involved in acute intermittent porphyria. Proper understanding of PPOX and its pathway interactions helps in managing and diagnosing such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

See full target information PPOX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPOX antibody [EPR10401] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Western blot - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (AB249564)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise