Anti-PPP1CA + PPP1CB antibody [EP1511Y]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

Overlay histogram showingHeLa cells stained with ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150077(1/500) an Alexa Fluor^®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

Overlay histogram showing HeLa cells stained with ab52619, unpurified (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• IP

Unknown

Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619)

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

All lanes:

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/20000 dilution

Lane 1:

SKBR-3 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

All lanes:

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution

Lane 1:

Mouse Brain lysate at 20 µg

Lane 2:

Rat Brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (AB52619)

All lanes:

Western blot - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution

Lane 1:

T47-D cell Lysate at 20 µg

Lane 2:

HeLa cell Lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 37 kDa

false